Independence of plasma membrane blebbing from other biochemical and biological characteristics of apoptotic cells

被引:27
作者
Shiratsuchi, A
Mori, T
Nakanishi, Y [1 ]
机构
[1] Kanazawa Univ, Grad Sch Med Sci, Kanazawa, Ishikawa 9200934, Japan
[2] Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9200934, Japan
关键词
apoptosis; membrane blebbing; phagocytosis; ROCK-I;
D O I
10.1093/oxfordjournals.jbchem.a003233
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plasma membrane blebs are observed in many types of apoptotic cells, but their physiological roles remain to be clarified. We examined whether there is a causative connection between membrane blebbing and other apoptotic changes in Jurkat cells induced to undergo apoptosis by doxorubicin in the presence or absence of Y-27632, an inhibitor of the Rho kinase ROCK-I. The inclusion of the drug made most membrane blebs disappear, while other changes, such as chromatin condensation, inactivation of mitochondrial enzymes, externalization of the membrane phospholipid phosphatidylserine, and removal of cell surface sialic acid, remained unaffected. Furthermore, these apoptotic cells were phagocytosed by macrophages as efficiently as normally apoptosing cells. These results indicate that blebbing of the plasma membrane occurs independently from other apoptotic changes and is not involved in the recognition and engulfment of apoptotic cells by macrophages.
引用
收藏
页码:381 / 386
页数:6
相关论文
共 46 条
[1]   La autoantigen is cleaved in the COOH terminus and loses the nuclear localization signal during apoptosis [J].
Ayukawa, K ;
Taniguchi, S ;
Masumoto, J ;
Hashimoto, S ;
Sarvotham, H ;
Hara, A ;
Aoyama, T ;
Sagara, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (44) :34465-34470
[2]   Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: Implications for coagulation events and antigenic spread in systemic lupus erythematosus [J].
CasciolaRosen, L ;
Rosen, A ;
Petri, M ;
Schlissel, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) :1624-1629
[3]   AUTOANTIGENS TARGETED IN SYSTEMIC LUPUS-ERYTHEMATOSUS ARE CLUSTERED IN 2 POPULATIONS OF SURFACE-STRUCTURES ON APOPTOTIC KERATINOCYTES [J].
CASCIOLAROSEN, LA ;
ANHALT, G ;
ROSEN, A .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (04) :1317-1330
[4]  
COHEN JJ, 1992, ANNU REV IMMUNOL, V10, P267, DOI 10.1146/annurev.iy.10.040192.001411
[5]   Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I [J].
Coleman, ML ;
Sahai, EA ;
Yeo, M ;
Bosch, M ;
Dewar, A ;
Olson, MF .
NATURE CELL BIOLOGY, 2001, 3 (04) :339-345
[6]   Involvement of p38 in apoptosis-associated membrane blebbing and nuclear condensation [J].
Deschesnes, RG ;
Huot, J ;
Valerie, K ;
Landry, J .
MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (06) :1569-1582
[7]   Germ cell apoptosis after treatment of cryptorchidism with human chorionic gonadotropin is associated with impaired reproductive function in the adult [J].
Dunkel, L ;
Taskinen, S ;
Hovatta, O ;
Tilly, JL ;
Wikstrom, S .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (09) :2341-2346
[8]   MECHANISMS AND FUNCTIONS OF CELL-DEATH [J].
ELLIS, RE ;
YUAN, JY ;
HORVITZ, HR .
ANNUAL REVIEW OF CELL BIOLOGY, 1991, 7 :663-698
[9]   A receptor for phosphatidylserine-specific clearance of apoptotic cells [J].
Fadok, VA ;
Bratton, DL ;
Rose, DM ;
Pearson, A ;
Ezekewitz, RAB ;
Henson, PM .
NATURE, 2000, 405 (6782) :85-90
[10]   The role of phosphatidylserine in recognition of apoptotic cells by phagocytes [J].
Fadok, VA ;
Bratton, DL ;
Frasch, SC ;
Warner, ML ;
Henson, PM .
CELL DEATH AND DIFFERENTIATION, 1998, 5 (07) :551-562