Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart

被引：38

作者：

Bradu, Adrian ^{[1
]}

Ma, Lisha ^{[2
]}

Bloor, James W. ^{[2
]}

Podoleanu, Adrian ^{[1
]}

机构：

[1] Univ Kent, Appl Opt Grp, Sch Phys Sci, Canterbury CT2 7NH, Kent, England

[2] Univ Kent, Cell & Dev Biol Grp, Dept Biosci, Canterbury CT2 7NJ, Kent, England

来源：

JOURNAL OF BIOPHOTONICS | 2009年 / 2卷 / 6-7期

基金：

英国生物技术与生命科学研究理事会;

关键词：

optical coherence tomography; laser scanning confocal microscopy; fluorescence; sequential imaging; Drosophila melanogaster; green fluorescent protein; heart; GENETIC-CONTROL; HIGH-SPEED; TOMOGRAPHY; MORPHOGENESIS; SYSTEM;

D O I：

10.1002/jbio.200910021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article demonstrates a combined instrument of two imaging modalities to acquire information, on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross-sectional OCT and C-scan LSFM images has been developed. With this dual-imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in cardiac cycle can be monitored. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：380 / 388

页数：9

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