Determination of species-specific sequences of superoxide dismutase A encoding gene sodA and chaperonin 60 encoding gene cpn60 for identification and phylogenetic analysis of Streptococcus phocae

被引：12

作者：

Alber, J

El-Sayed, A

Lämmler, C

Hassan, AA

Vossen, A

Siebert, U

机构：

[1] Univ Giessen, Inst Pharmakol & Toxikol, D-35392 Giessen, Germany

[2] Forsch & Technol Zentrum Westkuste, Hafentorn, D-25761 Busum, Germany

[3] Rhein Sieg Kreis, D-53721 Siegburg, Germany

[4] Univ Giessen, Inst Tierarztliche Nahrungsmittelkunde, D-35390 Giessen, Germany

来源：

VETERINARY MICROBIOLOGY | 2004年 / 101卷 / 02期

关键词：

Streptococcus phocae; superoxide dismutase A gene; chaperonin; 60; gene; polymerase chain reaction;

D O I：

10.1016/j.vetmic.2004.03.018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Species-specific PCR tests, based on the manganese-dependent superoxide dismutase A encoding gene (sodA) and the chaperonin 60 encoding gene (cpn60), were developed for the identification of Streptococcus phocae, a bacterial pathogen of seals. The selection of both oligonucleotide primer pairs was performed after amplification and sequencing of internal parts of both genes using universal oligonucleotide primers. The sequence studies of both genes additionally confirmed that S. phocae could taxonomically be classified to the pyogenic group of the genus Streptococcus. (C) 2004 Elsevier B.V. All rights reserved.

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页码：117 / 122

页数：6

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