Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization

被引：20

作者：

Leggett, JM ^{[1
]}

Perret, SJ ^{[1
]}

Harper, J ^{[1
]}

Morris, P ^{[1
]}

机构：

[1] Inst Grassland & Environm Res, Aberystwyth SY23 3EB, Dyfed, Wales

来源：

HEREDITY | 2000年 / 84卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Avena; cotransformation; FISH; oat transformation; physical mapping;

D O I：

10.1046/j.1365-2540.2000.00627.x

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Fluorescence in situ hybridization (FISH) was used to localize two transgenes (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mitotic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic callus. Among the eight progeny analysed by FISH in each of two lines, we detected plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our results demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chromosomes bearing a C genome translocation.

引用

页码：46 / 53

页数：8

共 20 条

[1] GENOMIC IN-SITU HYBRIDIZATION IN AVENA-SATIVA [J].

CHEN, QF ;

ARMSTRONG, K .

GENOME, 1994, 37 (04) :607-612

[2]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

[3] CLONING AND CHARACTERIZATION OF RIBOSOMAL-RNA GENES FROM WHEAT AND BARLEY [J].

GERLACH, WL ;

BEDBROOK, JR .

NUCLEIC ACIDS RESEARCH, 1979, 7 (07) :1869-1885

[4] Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots [J].

Kohli, A ;

Leech, M ;

Vain, P ;

Laurie, DA ;

Christou, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (12) :7203-7208

[5] THE GENOMIC IDENTIFICATION OF SOME MONOSOMICS OF AVENA-SATIVA L-CV SUN-II USING GENOMIC IN-SITU HYBRIDIZATION [J].

LEGGETT, JM ;

MARKHAND, GS .

GENOME, 1995, 38 (04) :747-751

[6] Discrimination of the closely related A and D genomes of the hexaploid oat Avena sativa L. [J].

Linares, C ;

Ferrer, E ;

Fominaya, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12450-12455

[7] ISOLATION OF AN EFFICIENT ACTIN PROMOTER FOR USE IN RICE TRANSFORMATION [J].

MCELROY, D ;

ZHANG, WG ;

CAO, J ;

WU, R .

PLANT CELL, 1990, 2 (02) :163-171

[8]

MICHAELS SD, 1994, BIOTECHNIQUES, V17, P274

[9] The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco [J].

Moscone, EA ;

Matzke, MA ;

Matzke, AJM .

CHROMOSOMA, 1996, 105 (04) :231-236

[10] Irregular patterns of transgene silencing in allohexaploid oat [J].

Pawlowski, WP ;

Torbert, KA ;

Rines, HW ;

Somers, DA .

PLANT MOLECULAR BIOLOGY, 1998, 38 (04) :597-607

← 1 2 →