Guanine nucleotides block agonist-driven 45Ca2+ influx in chick embryo retinal explants

被引：16

作者：

Burgos, JS ^{[1
]}

Barat, A ^{[1
]}

Ramírez, G ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Ctr Biol Mol, E-28049 Madrid, Spain

来源：

NEUROREPORT | 2000年 / 11卷 / 10期

关键词：

Ca2+-influx; chick retinal explants; glutamate antagonists; guanine nucleotides; ionotropic glutamate receptors; neuroprotection;

D O I：

10.1097/00001756-200007140-00047

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

AMPA (EC50=1.0 x 10(-6)M) and NMDA (EC50=1.3 x 10(-4)M) trigger Ca-45(2+) influx in 13-day chick embryonic retinal explants. This agonist-driven cationic flux is specifically inhibited by typical competitive antagonists, such as 6,7-dinitroquinoxaline-2,3-dione (DNQX) and 2-amino-7-phosphonoheptanoate (AP7), respectively. Guanine nucleotides, with different degrees of phosphorylation, namely 5'-GMP, guanosine 5'-O-(2-thiodiphosphate) (GDP beta S), guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and 5'-guanylyl-imidodiphosphate (GppNHp), are also efficient blockers of Ca-45(2+) influx. These results confirm the antagonistic behavior of guanine nucleotides towards ionotropic glutamate receptors and suggest a convenient experimental approach for screening of novel agonists and antagonists. NeuroReport 11:2303-2305 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：2303 / 2305

页数：3

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