Growth of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells on the Terpolyester Poly(3-hydroxybutyrate-co-3-hydroxyvalerateco-3-hydroxyhexanoate)

被引：18

作者：

Ji, Guang-Zhen ^{[1
]}

Wei, Xing ^{[1
]}

Chen, Guo-Qiang ^{[1
,2
]}

机构：

[1] Shantou Univ, Multidisciplinary Res Ctr, Shantou 515063, Guangdong, Peoples R China

[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, Minist Educ, Prot Sci Lab, Beijing 100084, Peoples R China

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2009年 / 20卷 / 03期

关键词：

Polyhydroxyalkanoates; PHBV; PHBHHx; PLA; mesenchymal stem cells; IN-VITRO; OSTEOBLAST ADHESION; SURFACE-ROUGHNESS; PROLIFERATION; FIBROBLAST; PROTEIN; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE); POLYHYDROXYALKANOATES; DIFFERENTIATION; MODEL;

D O I：

10.1163/156856209X412191

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

As a new member of the polyhydroxyalkanoate (PHA) family, the terpolyester poly(3-hydroxybutyrateco-3- hydroxyvalerate-co-3-hydroxyhexanoate) (PHBVHHx) was evaluated for its biocompatibility for human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs). More WJ-MSC adhesion and proliferation were observed on PHBVHHx film compared with films made of poly(L-lactic acid) (PLA), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Higher DNA synthesis by WJ-MSCs was detected on PHBVHHx film than on PLA, PHBV and PHBHHx films. PHBVHHx film had a rougher surface and more adsorption of extracellular matrix (ECM) proteins including collagen I, fibronectin and vitronectin, compared with PLA, PHBV and PHBHHx films. PHBVHHx film was also more hydrophobic than PLA and PHBV. These results demonstrated that PHBVHHx could be a promising biomaterial in medical implant applications for supporting the growth of cells, including WJ-MSCs. (c) Koninklijke Brill NV, Leiden, 2009

引用

页码：325 / 339

页数：15

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