Isolation and genotyping of Toxoplasma gondii from free-ranging chickens from Mexico

被引：51

作者：

Dubey, JP

Morales, ES

Lehmann, T

机构：

[1] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

[2] Ctr Dis Control & Prevent, Div Parasit Dis, Atlanta, GA 30341 USA

[3] Univ Nacl Autonoma Mexico, Fac Med Vet & Zooten, Dept Patol, Mexico City 04510, DF, Mexico

来源：

JOURNAL OF PARASITOLOGY | 2004年 / 90卷 / 02期

关键词：

D O I：

10.1645/GE-194R

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the presence of T. gondii oocysts in the environment because chickens feed from the soil. In the present study, prevalence of T. gondii in 208 free-range chickens (Gallus domesticus) from Mexico was investigated. Blood, heart, and brain from each animal were obtained to test for T. gondii infection. Antibodies to T gondii, assayed with the modified agglutination test (1:10 or higher), were found in 13 (6.2%) chickens. Hearts and brains of 13 seropositive chickens were bioassayed in mice, and T. gondii was isolated from 6 chickens. All 6 isolates were avirulent for mice. Genotyping of chicken isolates of T. gondii using the SAG2 locus indicated that 5 were type III and 1 was type I. This is the first report of isolation of T. gondii from chickens from Mexico.

引用

页码：411 / 413

页数：3

共 13 条

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