CCD video observation of microgravity crystallization: Apocrustacyanin C-1

被引：50

作者：

Chayen, NE

Snell, EH

Helliwell, JR

Zagalsky, PF

机构：

[1] Biophysics Section, Blackett Laboratory, Imperial College

[2] Chemistry Department, University of Manchester

[3] Biochemistry Department, Royal Holloway, University of London, Egham

[4] NASA, Laboratory for Structural Biology, Code ES76, Huntsville

来源：

JOURNAL OF CRYSTAL GROWTH | 1997年 / 171卷 / 1-2期

关键词：

D O I：

10.1016/S0022-0248(96)00382-X

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Apocrustacyanin C-1 has been crystallized in the vapour-diffusion apparatus of ESA's Advanced Protein Crystallization Facility (APCF) on-board the NASA space shuttle STS-65 International Microgravity Laboratory-2 (IML-2) mission. Crystal growth was monitored by black and white CCD observation at time intervals throughout the experiment. The resulting crystals displayed a motion within the hanging drop that is attributed to Marangoni convection effects. The images also show a ''halo'' effect around the growing crystals which can be attributed to the presence of depletion zones i.e. solution regions which are depleted of this coloured protein.

引用

页码：219 / 225

页数：7

共 12 条

[1] EXPERIMENT EQUIPMENT FOR PROTEIN CRYSTALLIZATION IN MU-G FACILITIES [J].