CCD video observation of microgravity crystallization: Apocrustacyanin C-1

被引:50
作者
Chayen, NE
Snell, EH
Helliwell, JR
Zagalsky, PF
机构
[1] Biophysics Section, Blackett Laboratory, Imperial College
[2] Chemistry Department, University of Manchester
[3] Biochemistry Department, Royal Holloway, University of London, Egham
[4] NASA, Laboratory for Structural Biology, Code ES76, Huntsville
关键词
D O I
10.1016/S0022-0248(96)00382-X
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
Apocrustacyanin C-1 has been crystallized in the vapour-diffusion apparatus of ESA's Advanced Protein Crystallization Facility (APCF) on-board the NASA space shuttle STS-65 International Microgravity Laboratory-2 (IML-2) mission. Crystal growth was monitored by black and white CCD observation at time intervals throughout the experiment. The resulting crystals displayed a motion within the hanging drop that is attributed to Marangoni convection effects. The images also show a ''halo'' effect around the growing crystals which can be attributed to the presence of depletion zones i.e. solution regions which are depleted of this coloured protein.
引用
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页码:219 / 225
页数:7
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