Pyrosequencing Reveals Contrasting Soil Bacterial Diversity and Community Structure of Two Main Winter Wheat Cropping Systems in China

被引:310
作者
Zhao, Jun [1 ,2 ]
Zhang, Ruifu [1 ,2 ,3 ]
Xue, Chao [1 ,2 ]
Xun, Weibing [1 ,2 ]
Sun, Li [1 ,2 ]
Xu, Yangchun [1 ,2 ,3 ]
Shen, Qirong [1 ,2 ,3 ]
机构
[1] Minist Agr, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Nanjing 210095, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Jiangsu Key Lab & Engn Ctr Solid Organ Waste Util, Nanjing 210095, Jiangsu, Peoples R China
[3] Jiangsu Collaborat Innovat Ctr Solid Organ Waste, Nanjing 210095, Jiangsu, Peoples R China
关键词
16S RIBOSOMAL-RNA; MICROBIAL COMMUNITY; FUNGAL COMMUNITIES; LAND-USE; RHIZOSPHERE; GRADIENT; MANAGEMENT; ROTATION; TILLAGE; PH;
D O I
10.1007/s00248-013-0322-0
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071301 [植物生态学];
摘要
Microbes are key components of the soil environment, playing an important role in maintaining soil health, sustainability, and productivity. The composition and structure of soil bacterial communities were examined in winter wheat-rice (WR) and winter wheat-maize (WM) cropping systems derived from five locations in the Low-Middle Yangtze River plain and the Huang-Huai-Hai plain by pyrosequencing of the 16S ribosomal RNA gene amplicons. A total of 102,367 high quality sequences were used for multivariate statistical analysis and to test for correlation between community structure and environmental variables such as crop rotations, soil properties, and locations. The most abundant phyla across all soil samples were Proteobacteria, Acidobacteria, and Bacteroidetes. Similar patterns of bacterial diversity and community structure were observed within the same cropping systems, and a higher relative abundance of anaerobic bacteria was found in WR compared to WM cropping systems. Variance partitioning analysis revealed complex relationships between bacterial community and environmental variables. The effect of crop rotations was low but significant, and interactions among soil properties, locations, and crop rotations accounted for most of the explained variation in the structure of bacterial communities. Soil properties such as pH, available P, and available K showed higher correlations (positive or negative) with the majority of the abundant taxa. Bacterial diversity (the Shannon index) and richness (Chao1 and ACE) were higher under WR than WM cropping systems.
引用
收藏
页码:443 / 453
页数:11
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