The expression of α-, β-, and γ-synucleins in olfactory mucosa from patients with and without neurodegenerative diseases

被引:104
作者
Duda, JE [1 ]
Shah, U
Arnold, SE
Lee, VMY
Trojanowski, JQ
机构
[1] Univ Penn, Sch Med, Dept Pathol & Lab Med, Ctr Neurodegenerat Dis Res, Philadelphia, PA 19104 USA
[2] Univ Penn, Sch Med, Dept Psychiat, Philadelphia, PA 19104 USA
[3] Univ Penn, Sch Med, Dept Neurol, Philadelphia, PA 19104 USA
基金
美国国家卫生研究院;
关键词
synuclein proteins; olfactory epithelium; Alzheimer's disease; Parkinson's disease; multiple system atrophy; synucleinopathies; regeneration; neuronal plasticity;
D O I
10.1006/exnr.1999.7228
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA). Since olfactory dysfunction is a common feature of these disorders and the olfactory receptor neurons (ORNs) of the olfactory epithelium (OE) regenerate throughout the lifespan, we used antibodies specific for alpha-, beta-, and gamma-synucleins to examine the olfactory mucosa of patients with PD, DLB, AD, MSA, and controls without a neurological disorder. Although antibodies to alpha- and beta-synucleins detected abnormal dystrophic neurites in the OE of patients with neurodegenerative disorders, similar pathology was also seen in the OE of controls. More significantly, we show here for the first time that alpha-, beta-, and gamma-synucleins are differentially expressed in cells of the OE and respiratory epithelium and that alpha-synuclein is the most abundant synuclein in the olfactory mucosa, where it is prominently expressed in ORNs. Moreover, alpha- and gamma-synucleins also were prominent in the OE basal cells, which include the progenitor cells of the ORNs in the OE. Thus, our data on synuclein expression within the OE may signify that synuclein plays a role in the regeneration and plasticity of ORNs in the adult human OE. (C) 1999 Academic Press.
引用
收藏
页码:515 / 522
页数:8
相关论文
共 40 条
  • [1] The synucleins: a family of proteins involved in synaptic function, plasticity, neurodegeneration and disease
    Clayton, DF
    George, JM
    [J]. TRENDS IN NEUROSCIENCES, 1998, 21 (06) : 249 - 254
  • [2] BETA-AMYLOID PEPTIDE AND AMYLOID PRECURSOR PROTEINS IN OLFACTORY MUCOSA OF PATIENTS WITH ALZHEIMERS-DISEASE, PARKINSONS-DISEASE, AND DOWN-SYNDROME
    CRINO, PB
    MARTIN, JA
    HILL, WD
    GREENBERG, B
    LEE, VMY
    TROJANOWSKI, JQ
    [J]. ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1995, 104 (08) : 655 - 661
  • [3] PRESENCE OF BOTH ODOR IDENTIFICATION AND DETECTION DEFICITS IN ALZHEIMERS-DISEASE
    DOTY, RL
    REYES, PF
    GREGOR, T
    [J]. BRAIN RESEARCH BULLETIN, 1987, 18 (05) : 597 - 600
  • [4] THE OLFACTORY AND COGNITIVE DEFICITS OF PARKINSONS-DISEASE - EVIDENCE FOR INDEPENDENCE
    DOTY, RL
    RIKLAN, M
    DEEMS, DA
    REYNOLDS, C
    STELLAR, S
    [J]. ANNALS OF NEUROLOGY, 1989, 25 (02) : 166 - 171
  • [5] CHARACTERIZATION OF A NOVEL PROTEIN REGULATED DURING THE CRITICAL PERIOD FOR SONG LEARNING IN THE ZEBRA FINCH
    GEORGE, JM
    JIN, H
    WOODS, WS
    CLAYTON, DF
    [J]. NEURON, 1995, 15 (02) : 361 - 372
  • [6] Mutant and wild type human α-synucleins assemble into elongated filaments with distinct morphologies in vitro
    Giasson, BI
    Uryu, K
    Trojanowski, JQ
    Lee, VMY
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (12) : 7619 - 7622
  • [7] Consensus statement on the diagnosis of multiple system atrophy
    Gilman, S
    Low, PA
    Quinn, N
    Albanese, A
    Ben-Shlomo, Y
    Fowler, CJ
    Kaufman, H
    Klockgether, T
    Lang, AE
    Lantos, PL
    Litvan, I
    Mathias, CJ
    Oliver, E
    Robertson, D
    Schatz, I
    Wenning, GK
    [J]. JOURNAL OF THE NEUROLOGICAL SCIENCES, 1999, 163 (01) : 94 - 98
  • [8] Hsu LJ, 1998, J NEUROCHEM, V71, P338
  • [9] Degenerative terminals of the perforant pathway are human α-synuclein-immunoreactive in the hippocampus of patients with diffuse Lewy body disease
    Iseki, E
    Marui, W
    Kosaka, K
    Akiyama, H
    Ueda, K
    Iwatsubo, T
    [J]. NEUROSCIENCE LETTERS, 1998, 258 (02) : 81 - 84
  • [10] Jacobson, 1978, HDB SENSORY PHYSL, V9