A genetically encoded infrared probe

被引：212

作者：

Schultz, Kathryn C.

Supekova, Lubica

Ryu, Youngha

Xie, Jianming

Perera, Roshan

Schultz, Peter G.

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 43期

关键词：

D O I：

10.1021/ja0636690

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes. Copyright © 2006 American Chemical Society.

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页码：13984 / 13985

页数：2

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