Identification and characterization of the high-affinity choline transporter

被引：269

作者：

Okuda, T ^{[1
]}

Haga, T

Kanai, Y

Endou, H

Ishihara, T

Katsura, I

机构：

[1] Univ Tokyo, Fac Med, Dept Neurochem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130033, Japan

[3] Kyorin Univ, Sch Med, Dept Pharmacol & Toxicol, Tokyo 1818611, Japan

[4] Japan Sci & Technol Corp, PRESTO, Tokyo 1818611, Japan

[5] Grad Univ Adv Studies, Sch Life Sci, Dept Genet, Mishima, Shizuoka 4118540, Japan

[6] Grad Univ Adv Studies, Natl Inst Genet, Struct Biol Ctr, Mishima, Shizuoka 4118540, Japan

来源：

NATURE NEUROSCIENCE | 2000年 / 3卷 / 02期

基金：

日本学术振兴会;

关键词：

D O I：

10.1038/72059

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho-1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

引用

页码：120 / 125

页数：6

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