Skin biopsy as a nondestructive tool for the toxicological assessment of endangered populations of pinnipeds: Preliminary results on mixed function oxidase in Otaria flavescens

被引：22

作者：

Fossi, MC

Savelli, C

Marsili, L

Casini, S

Jimenez, B

Junin, M

Castello, H

Lorenzani, JA

机构：

[1] ARGENTINE INST NAT SCI BERNARDINO RIVADAVIA,RA-1405 BUENOS AIRES,DF,ARGENTINA

[2] FDN FAUNA ARGENTINA,RA-7600 MAR DEL PLATA,ARGENTINA

来源：

CHEMOSPHERE | 1997年 / 35卷 / 08期

关键词：

pinnipeds; skin biopsy; nondestructive biomarkers; OCs; PAHs;

D O I：

10.1016/S0045-6535(97)00244-0

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The aim of this study was to develop and validate a method for assessing the toxicological risk of endangered populations of pinnipeds based on a nondestructive biological tool, the skin biopsy specimen. Skin biopsies can be obtained from pinnipeds by anaesthetising the animals and taking a small amount of skin in the anterior flipper area, or by shooting a biopsy dart with a crossbow. Skin biopsy material is suitable for a wide range of chemical and biomarker analysis. Organochlorines and polycyclic aromatic hydrocarbons can be analysed in subcutaneous fat and MFO activity (BPMO), Cyt.P450 isoforms, and DNA damage can be detected in epidermis. (C) 1997 Elsevier Science Ltd.

引用

页码：1623 / 1635

页数：13

共 38 条

[1] CHARACTERIZATION OF ETHOXYRESORUFIN O-DE-ETHYLASE IN GREY SEAL HALICHOERUS-GRYPUS [J].