Polarization of chemoattractant receptor signaling during neutrophil chemotaxis

被引：700

作者：

Servant, G

Weiner, OD

Herzmark, P

Balla, T

Sedat, JW

Bourne, HR ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

[3] NICHHD, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2000年 / 287卷 / 5455期

关键词：

D O I：

10.1126/science.287.5455.1037

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Morphologic polarity is necessary for chemotaxis of mammalian cells. As a probe of intracellular signals responsible for this asymmetry, the pleckstrin homology domain of the AKT protein kinase (or protein kinase B), tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils. Upon exposure of cells to chemoattractant, PHAKT-GFP is recruited selectively to membrane at the cell's leading edge, indicating an internal signaling gradient that is much steeper than that of the chemoattractant. Translocation of PHAKT-GFP is inhibited by toxin-B from Clostridium difficile, indicating that it requires activity of one or more Rho guanosine triphosphatases.

引用

页码：1037 / 1040

页数：4

共 48 条

[1] ACTIVATION OF THE NADPH OXIDASE INVOLVES THE SMALL GTP-BINDING PROTEIN P21RAC1 [J].