Molecular anatomy of a trafficking organelle

被引：1743

作者：

Takamori, Shigeo

Holt, Matthew

Stenius, Katinka

Lemke, Edward A.

Gronborg, Mads

Riedel, Dietmar

Urlaub, Henning

Schenck, Stephan

Bruegger, Britta

Ringler, Philippe

Mueller, Shirley A.

Rammner, Burkhard

Graeter, Frauke

Hub, Jochen S.

De Groot, Bert L.

Mieskes, Gottfried

Moriyama, Yoshinori

Klingauf, Juergen

Grubmueller, Helmut

Heuser, John

Wieland, Felix

Jahn, Reinhard ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Dept Membrane Biophys, D-37077 Gottingen, Germany

[3] Max Planck Inst Biophys Chem, Electron Microscopy Lab, D-37077 Gottingen, Germany

[4] Max Planck Inst Biophys Chem, Lab Bioanalyt Mass Spectrometry, D-37077 Gottingen, Germany

[5] Max Planck Inst Biophys Chem, Lab Biomol Dynam, D-37077 Gottingen, Germany

[6] Max Planck Inst Biophys Chem, Dept Theoret & Computat Biophys, D-37077 Gottingen, Germany

[7] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA

[8] Heidelberg Univ, Dept Biochem, D-69120 Heidelberg, Germany

[9] Univ Basel, Bioctr, Maurice E Muller Inst High Resolut Electron M, CH-4056 Basel, Switzerland

[10] Scimotion, D-22765 Hamburg, Germany

[11] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Lab Membrane Biochem, Okayama 7008530, Japan

[12] Washington Univ, Dept Cell Biol, St Louis, MO 63110 USA

来源：

CELL | 2006年 / 127卷 / 04期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/j.cell.2006.10.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.

引用

页码：831 / 846

页数：16

共 63 条

[1] Regulation of vesicular neurotransmitter transporters [J].