Genetically selected stem cells from human adipose tissue express cardiac markers

被引：51

作者：

Bai, Xiaowen

Pinkernell, Kai

Song, Yao-Hua

Nabzdyk, Christoph

Reiser, Jakob

Alt, Eckhard

机构：

[1] Univ Texas, MD Anderson Canc Ctr, Dept Mol Pathol, Houston, TX 77030 USA

[2] Tulane Univ, Hlth Sci Ctr, Dept Med, Cardiol Sect, New Orleans, LA 70112 USA

[3] Tulane Univ, Hlth Sci Ctr, Dept Microbiol Immunol & Parasitol, New Orleans, LA 70112 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 353卷 / 03期

关键词：

cardiomyocytes; adipose-derived stem cell; differentiation;

D O I：

10.1016/j.bbrc.2006.12.103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, the potential of human adipose-derived stem cells to differentiate into cells with characteristics of cardiomyocytes was investigated. Adipose tissue-derived stem cells (ADSCs) were transduced with two different lentiviral vectors simultaneously: (1) a lentiviral vector expressing eGFP controlled by the Nkx2.5 promoter and (2) a lentiviral vector expressing DsRed2 controlled by the myosin light chain-2v promoter (MLC-2v). Nkx2.5-eGFP and MLC-2v-DsRed2 dual positive cells were isolated by FACS. Immuno-staining and RT-PCR analysis of the dual positive cells revealed that these cells are positive for Nkx2.5, cardiac troponin I, and L-type calcium channel alpha-1c subunit. Electrophysiology studies demonstrated the presence of functional voltage-dependent calcium and potassium channels. These observations confirm that cardiac progenitor cells can be isolated and enriched from human adipose-derived stem cells using lentiviral selection, and they might represent a new source for cell therapy for myocardial infarction and heart failure. (c) 2006 Elsevier Inc. All rights reserved.

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页码：665 / 671

页数：7

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