Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity

被引：3

作者：

Arroyo, M ^{[1
]}

Torres-Guzmán, R ^{[1
]}

Torres-Bacete, J ^{[1
]}

de la Mata, I ^{[1
]}

Castillón, MP ^{[1
]}

Acebal, C ^{[1
]}

机构：

[1] Univ Complutense, Fac Ciencias Biol, Dept Bioquim & Biol Mol 1, E-28040 Madrid, Spain

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 13期

关键词：

chromogenic substrates; penicillin acylase; Streptomyces lavendulae;

D O I：

10.1023/A:1016096111453

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The synthesis of 2-nitro-5-[(hexanoyl)-amino]-benzoic acid and 2-nitro-5-[(octanoyl)-amino]-benzoic acid as chromogenic substrates for the determination of aliphatic penicillin acylase activity is described. During enzymatic hydrolysis, the released chromophore, 2-nitro-5-amino-benzoic acid, was detected at 405 nm. Penicillin acylase from Streptomyces lavendulae had an appreciable activity towards these substrates, which can then be used to detect penicillin acylases able to cleave hexanoyl and octanoyl residues off synthetic amides as well as penicillin dihydroF and penicillin K, their natural analogues.

引用

页码：1045 / 1048

页数：4

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