Deep-etching electron microscopy of cells of Magnetospirillum magnetotacticum:: Evidence for filamentous structures connecting the magnetosome chain to the cell surface

被引:16
作者
Martins, Juliana Lopes
Keim, Carolina Neumann
Farina, Marcos
Kachar, Bechara
Lins, Ulysses
机构
[1] Univ Fed Rio de Janeiro, CCS, Dept Microbiol Geral, Inst Microbiol Prof Paulo de Goes, BR-21941590 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, CCS, Inst Ciencias Biomed, BR-21941590 Rio De Janeiro, Brazil
[3] NIDCD, Sect Struct Cell Biol, NIH, Bethesda, MD 20892 USA
关键词
MAGNETITE; ULTRASTRUCTURE; MEMBRANE;
D O I
10.1007/s00284-005-0221-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Magnetospirillum magnetotacticum are magnetotactic bacteria that form a single chain of magnetite magnetosomes within its cytoplasm. Here, we studied the ultrastructure of M. magnetotacticum by freeze-fracture and deep-etching to understand the spatial correlation between the magnetosome chain and the cell envelope and its possible implications for magnetotaxis. Magnetosomes were found mainly near the cell envelope, forming chains that were closely associated with the granular cytoplasmic material. The membrane surrounding the magnetosomes could be visualized in deep-etching preparations. Thin connections between magnetosome chains and the cell envelope were observed in deep-etching images. These results strengthen the hypothesis for the existence of structures that transfer the torque from the magnetosome chains to the whole cell during the orientation of magnetotactic bacteria to a magnetic field lines.
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页码:1 / 4
页数:4
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