Effect of cell density on adipogenic differentiation of mesenchymal stem cells

被引：50

作者：

Lu, Hongxu ^{[1
,2
]}

Guo, Likun ^{[1
,3
]}

Wozniak, Michal J. ^{[1
]}

Kawazoe, Naoki ^{[1
,4
]}

Tateishi, Tetsuya ^{[1
]}

Zhang, Xingdong ^{[3
]}

Chen, Guoping ^{[1
,2
,4
]}

机构：

[1] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050044, Japan

[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058577, Japan

[3] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610064, Peoples R China

[4] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton MANA, Tsukuba, Ibaraki 3050044, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 03期

关键词：

Mesenchymal stem cells; Adipogenic differentiation; Cell density; Micropattern; Tissue engineering; ADULT BONE-MARROW; CHONDROGENIC DIFFERENTIATION; COLLAGEN SCAFFOLDS; CULTURE-CONDITIONS; IN-VITRO; EXPANSION; ADIPOCYTE; SURFACES; LINEAGE;

D O I：

10.1016/j.bbrc.2009.01.174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The effect of cell density on the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) was investigated by using a patterning technique to induce the formation of a cell density gradient on a micropatterned Surface. The adipogenic differentiation of MSCs at a density gradient from 5 x 10(3) to 3 x 10(4) cells/cm(2) was examined. Lipid vacuoles were observed at all cell densities after 1-3 weeks of culture in adipogenic differentiation medium although the lipid vacuoles were scarce at the low cell density and abundant at the high cell density. Real-time RT-PCR analysis showed that adipogenesis marker genes encoding peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2), lipoprotein lipase (LPL), and fatty acid binding protein-4 (FABP4) were detected in the MSCs cultured at all cell densities. The results suggest that there was no apparent effect of cell density on the adipogenic differentiation of human MSCs. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：322 / 327

页数：6

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