Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP-dependent K+ channel, ROMK6 (Kir1.1f)

被引：39

作者：

Kondo, C ^{[1
]}

Isomoto, S ^{[1
]}

Matsumoto, S ^{[1
]}

Yamada, M ^{[1
]}

Horio, Y ^{[1
]}

Yamashita, S ^{[1
]}

TakemuraKameda, K ^{[1
]}

Matsuzawa, Y ^{[1
]}

Kurachi, Y ^{[1
]}

机构：

[1] OSAKA UNIV,FAC MED,DEPT PHARMACOL 2,SUITA,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1996年 / 399卷 / 1-2期

关键词：

cloning; inwardly rectifying K+ channel; ROMK channel; splice variant; reverse transcription polymerase chain reaction; Xenopus oocyte;

D O I：

10.1016/S0014-5793(96)01302-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified from rat kidney a novel isoform of ROMK/Kir1.1, designated ROMK6/Kir1.1f. ROMK6 was nearly identical to ROMK1, but possessed an 122-bp insertion in the 5' region. Its deduced amino acid sequence was shorter by 19 amino acids than that of ROMK1 in the amino-terminus. Unlike other previously reported ROMK isoforms, ROMK6 mRNA was ubiquitously expressed in various tissues, including kidney, brain, heart, liver, pancreas and skeletal muscle. Xenopus oocytes injected with ROMK6 cRNA expressed a Ba2+-sensitive weakly inwardly rectifying K+ current. These results indicate that ROMK6 is a novel functional K+ channel and might be involved in K+ secretion in various tissues.

引用

页码：122 / 126

页数：5

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