Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

被引：25

作者：

Moody, EM

Brown, TS

Bevilacqua, PC ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 33期

关键词：

D O I：

10.1021/ja047362h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids. Copyright © 2004 American Chemical Society.

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页码：10200 / 10201

页数：2

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