Purification, structure determination and synthesis of covalitoxin-II, a short insect-specific neurotoxic peptide from the venom of the Coremiocnemis validus (Singapore tarantula)

被引:19
作者
Balaji, RA
Sasaki, T
Gopalakrishnakone, P
Sato, K
Kini, RM
Bay, BH
机构
[1] Natl Univ Singapore, Fac Med, Dept Anat, Venom & Toxin Res Programme, Singapore 117597, Singapore
[2] Mitsubishi Kasei Inst Life Sci, Machida, Tokyo 1948511, Japan
[3] Natl Univ Singapore, Fac Sci, Biosci Ctr, Singapore 119260, Singapore
来源
FEBS LETTERS | 2000年 / 474卷 / 2-3期
关键词
spider; spider venom; reversible neurotoxin; Conus toxin; cystine motif;
D O I
10.1016/S0014-5793(00)01593-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Spider venoms contain toxins that specifically immobilize and kill insects. We report the purification and characterization of a new insect-specific toxin named covalitoxin-II (Cvtx-II; mass, 3306.24+/-0.64), from Coremiocnemis validus (Singapore tarantula) venom. The complete 31 amino acid sequence of Cvtx-II has been determined and it shows less than 40% identity with spider toxins, However, Cvtx-II has conserved cystine motif analogous to other spider and omega-conotoxsins. Cvtxs-II was chemically synthesized and identified with the native Cvtx-II, Synthetic Cvtx-II induced insect-specific non-lethal excitatory activity when injected into crickets, but not in cockroaches and mice, (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:208 / 212
页数:5
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