Instrumentation for chemical cytometry

被引：128

作者：

Krylov, SN ^{[1
]}

Starke, DA ^{[1
]}

Arriaga, EA ^{[1
]}

Zhang, ZR ^{[1
]}

Chan, NWC ^{[1
]}

Palcic, MM ^{[1
]}

Dovichi, NJ ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 04期

关键词：

D O I：

10.1021/ac991096m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis is ideally suited to chemical analysis of individual cells. Small mammalian somatic cells (similar to 1.5 mu m in diameter) can be analyzed by injecting the intact cell into a capillary, lysing the cell, separating and detecting the cellular components, and reconditioning the capillary prior to the next injection. In this paper, we report on technical improvements to single-cell analysis. We designed an inexpensive multipurpose single-cell injector that facilitates the following: (i) monitoring of injection, (ii) reproducible pressure- or electrokinetic-driven injection of the cell, (iii) complete cell lysis by SDS within 30 s of injection, and (iv) pressure-driven capillary reconditioning. Furthermore, we report on the analysis of glycosylation and glycolysis in single human carcinoma cells (HT29 cell line). The reliability and quality of the analysis is confirmed by comparing electropherograms from single cells and those from purified cell extracts.

引用

页码：872 / 877

页数：6

共 27 条

[1] Dilation of the influenza hemagglutinin fusion pore revealed by the kinetics of individual cell-cell fusion events [J].