Papain digestion of different mouse IgG subclasses as studied by electrospray mass spectrometry

被引：42

作者：

Adamczyk, M ^{[1
]}

Gebler, JC ^{[1
]}

Wu, J ^{[1
]}

机构：

[1] Abbott Labs, Abbott Diagnost Div, Dept Chem 9NM, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 237卷 / 1-2期

关键词：

IgG; papain digestion; subclass; electrospray mass spectrometry;

D O I：

10.1016/S0022-1759(00)00135-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

On-line liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) has been utilized to monitor the papain digestion of mouse monoclonal antibodies (mAb) of different subclasses (IgG1, IgG2a, IgG2b, and IgG3). The method is simple, rapid, sensitive, and allows for simultaneous determination of digestion products and the identification of microheterogenous fragments. The results provide important insight into the mechanism of papain digestion of mouse IgGs of different subclasses. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 104

页数：10

共 33 条

[1] Akashi S, 1996, J AM SOC MASS SPECTR, V7, P707, DOI 10.1016/1044-0305(96)80517-4
[2] [Anonymous], 1996, Monoclonal Antibodies: principes and practice
[3] MASS-SPECTROMETRY OF THE HUMANIZED MONOCLONAL-ANTIBODY CAMPATH 1H
ASHTON, DS
BEDDELL, CR
COOPER, DJ
CRAIG, SJ
LINES, AC
OLIVER, RWA
SMITH, MA
[J]. ANALYTICAL CHEMISTRY, 1995, 67 (05) : 835 - 842
[4] Rapid characterization of chemically-modified proteins by electrospray mass spectrometry
Bennett, KL
Smith, SV
Lambrecht, RM
Truscott, RJW
Sheil, MM
[J]. BIOCONJUGATE CHEMISTRY, 1996, 7 (01) : 16 - 22
[5] Monitoring papain digestion of a monoclonal antibody by electrospray ionization mass spectrometry
Bennett, KL
Smith, SV
Truscott, RJW
Sheil, MM
[J]. ANALYTICAL BIOCHEMISTRY, 1997, 245 (01) : 17 - 27
[6] Bloom JW, 1997, PROTEIN SCI, V6, P407
[7] IMPROVED PROCEDURE FOR PREPARATION OF F(AB')2 FRAGMENTS OF MOUSE IGGS BY PAPAIN DIGESTION
BOGUSLAWSKI, SJ
LEDDEN, DJ
FREDRICKSON, RA
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 120 (01) : 51 - 56
[8] ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF RECOMBINANTLY ENGINEERED ANTIBODY FRAGMENTS
BOURELL, JH
CLAUSER, KP
KELLEY, R
CARTER, P
STULT, JT
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (13) : 2088 - 2095
[9] DIFFERENTIAL ENZYME SUSCEPTIBILITY OF BOVINE IMMUNOGLOBULIN-G1 AND IMMUNOGLOBULIN-G2 TO PEPSIN AND PAPAIN
BUTLER, JE
KENNEDY, N
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 535 (01) : 125 - 137
[10] MOUSE IGG2B MONOCLONAL-ANTIBODY FRAGMENTATION - PREPARATION AND PURIFICATION OF FAB, FC AND FAB/C FRAGMENTS
DEMIGNOT, S
GARNETT, MC
BALDWIN, RW
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 121 (02) : 209 - 217

← 1 2 3 4 →