Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria

被引:20
作者
Fife, DJ [1 ]
Bruhn, DF [1 ]
Miller, KS [1 ]
Stoner, DL [1 ]
机构
[1] Idaho Natl Lab, Idaho Falls, ID 83415 USA
关键词
D O I
10.1128/AEM.66.5.2208-2210.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245-2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.
引用
收藏
页码:2208 / 2210
页数:3
相关论文
共 33 条
  • [1] BALASHOVA VV, 1974, MICROBIOLOGY+, V43, P491
  • [2] BRIERLEY JA, 1978, APPL ENVIRON MICROB, V36, P523, DOI 10.1128/AEM.36.3.523-525.1978
  • [3] SULFOLOBUS - NEW GENUS OF SULFUR-OXIDIZING BACTERIA LIVING AT LOW PH AND HIGH-TEMPERATURE
    BROCK, TD
    BROCK, KM
    BELLY, RT
    WEISS, RL
    [J]. ARCHIV FUR MIKROBIOLOGIE, 1972, 84 (01): : 54 - &
  • [4] CLARK DA, 1996, MICROBIOLOGY, V142, P1
  • [5] DESILONIZ MI, 1992, ARCH MICROBIOL, V159, P237
  • [6] Edwards KJ, 1999, GEOMICROBIOL J, V16, P155
  • [7] Edwards KJ, 1999, APPL ENVIRON MICROB, V65, P3627
  • [8] GHAURI MA, 1991, FEMS MICROBIOL ECOL, V85, P327
  • [9] CULTURAL AND PHYLOGENETIC ANALYSIS OF MIXED MICROBIAL-POPULATIONS FOUND IN NATURAL AND COMMERCIAL BIOLEACHING ENVIRONMENTS
    GOEBEL, BM
    STACKEBRANDT, E
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (05) : 1614 - 1621
  • [10] GOLOVACHEVA RS, 1978, MICROBIOLOGY+, V47, P658