Human cytomegalovirus (HCMV) UL82 gene product (pp71) relieves hDaxx-mediated repression of HCMV replication

被引：103

作者：

Cantrell, Stacy R. ^{[1
]}

Bresnahan, Wade A. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA

来源：

JOURNAL OF VIROLOGY | 2006年 / 80卷 / 12期

关键词：

D O I：

10.1128/JVI.02676-05

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study examines the role of the cellular protein hDaxx in controlling human cytomegalovirus (HCMV) immediate-early (IE) gene expression and viral replication. Using permissive cell lines that either overexpress hDaxx or are depleted of hDaxx expression by the use of short hairpin RNA, we demonstrate that hDaxx functions as a repressor of HCW IE gene expression and replication. In addition, we demonstrate that the impaired growth phenotype associated with the UL82 (pp71) deletion mutant is abolished when hDaxx knockdown cells are infected, suggesting that pp7l functions to relieve hDaxx-mediated repression during HCMV infection.

引用

页码：6188 / 6191

页数：4

共 35 条

[1] UL82 virion protein activates expression of immediate early viral genes in human cytomegalovirus-infected cells [J].

Bresnahan, WA ;

Shenk, TE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14506-14511

[2] Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication [J].

Cantrell, SR ;

Bresnahan, WA .

JOURNAL OF VIROLOGY, 2005, 79 (12) :7792-7802

[3] Transcriptional regulation of the human cytomegalovirus US11 early gene [J].

Chau, NH ;

Vanson, CD ;

Kerry, JA .

JOURNAL OF VIROLOGY, 1999, 73 (02) :863-870

[4] Daxx silencing sensitizes cells to multiple apoptotic pathways [J].

Chen, LY ;

Chen, JD .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (20) :7108-7121

[5] Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling [J].

Doucas, V ;

Tini, M ;

Egan, DA ;

Evans, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :2627-2632

[6] The cellular protein Daxx interacts with avian sarcoma virus integrase and viral DNA to repress viral transcription [J].

Greger, JG ;

Katz, RA ;

Ishov, AM ;

Maul, GG ;

Skalka, AM .

JOURNAL OF VIROLOGY, 2005, 79 (08) :4610-4618

[7] Functional interaction between the pp71 protein of human cytomegalovirus and the PML-interacting protein human Daxx [J].

Hofmann, H ;

Sindre, H ;

Stamminger, T .

JOURNAL OF VIROLOGY, 2002, 76 (11) :5769-5783

[8] The Pax3-FKHR oncoprotein is unresponsive to the Pax3-associated repressor hDaxx [J].

Hollenbach, AD ;

Sublett, JE ;

McPherson, CJ ;

Grosveld, G .

EMBO JOURNAL, 1999, 18 (13) :3702-3711

[9]

Hollenbach AD, 2002, J CELL SCI, V115, P3319

[10] Activation of herpesvirus gene expression by the human cytomegalovirus protein pp71 [J].

Homer, EG ;

Rinaldi, A ;

Nicholl, MJ ;

Preston, CM .

JOURNAL OF VIROLOGY, 1999, 73 (10) :8512-8518

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