Effects of androgen manipulation on α1-adrenoceptor subtypes in the rat seminal vesicle

被引：21

作者：

Mendes, FR ^{[1
]}

Hamamura, M ^{[1
]}

Queiróz, DBC ^{[1
]}

Porto, CS ^{[1
]}

Avellar, MCW ^{[1
]}

机构：

[1] Escola Paulista Med, UNIFESP, Sect Expt Endocrinol, Dept Pharmacol, BR-04044020 Sao Paulo, Brazil

来源：

LIFE SCIENCES | 2004年 / 75卷 / 12期

基金：

巴西圣保罗研究基金会;

关键词：

seminal vesicle; alpha(1)-adrenoceptor; castration; androgen;

D O I：

10.1016/j.lfs.2004.03.011

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This study analyses possible changes during surgical and chemical castration in the expression and pharmacological characteristics of alpha(1)-adrenoceptor in adult rat seminal vesicle. Ribonuclease protection assays indicated that alpha(1a)- was the predominant mRNA, while alpha(1b)- and alpha(1d)-adrenoceptor transcripts were detected in lower abundance in this tissue. alpha(1a)-adrenoceptor mRNA expression presented a complex dependency on androgens, while alpha(1b)- and alpha(1d)-adrenoceptor transcripts were both upregulated with surgical and chemical castration, suggesting a negative modulation by androgens. Testosterone treatment reversed the effects caused by surgical castration. Functional studies confirmed the involvement of alpha(1A)- and alpha(1B)-adrenoceptor in the seminal vesicle contractile responses, and suggested that alpha(1B)-induced contractile response was upregulated after castration. Taken together, the results suggest that alpha(1)-adrenoceptor expression in seminal vesicle is differentially regulated by the androgen status of the rat. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1449 / 1463

页数：15

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