Protein microchips: Use for immunoassay and enzymatic reactions

被引:419
作者
Arenkov, P
Kukhtin, A
Gemmell, A
Voloshchuk, S
Chupeeva, V
Mirzabekov, A
机构
[1] Argonne Natl Lab, Biochip Technol Ctr, Argonne, IL 60439 USA
[2] Russian Acad Sci, Engelhardt Inst Mol Biol, Joint Human Genome Program, Moscow 117984, Russia
关键词
fluorescence detection; immunoassay; antigen; antibody; enzyme; microchips;
D O I
10.1006/abio.1999.4363
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Different proteins such as antibodies, antigens, and enzymes were immobilized within the 100 x 100 x 20-mu m gel pads of protein microchips. A modified polyacrylamide gel has been developed to accommodate proteins of a size up to 400,000 daltons, Electrophoresis in the microchip reaction chamber speeded up antigen-antibody interactions within the gel. Protein microchips were used in immunoassays for detection of antigens or antibodies, as well as to carry out enzymatic reactions and to measure their kinetics in the absence or presence of an inhibitor. A protein microchip can be used several times in different immunoassays and enzymatic kinetic measurements. (C) 2000 Academic Press.
引用
收藏
页码:123 / 131
页数:9
相关论文
共 19 条
[1]  
COONS AH, 1950, J EXP MED, V91, P1, DOI 10.1084/jem.91.1.1
[2]   Sequence analysis by hybridization with oligonucleotide microchip: Identification of beta-thalassemia mutations [J].
Drobyshev, A ;
Mologina, N ;
Shik, V ;
Pobedimskaya, D ;
Yershov, G ;
Mirzabekov, A .
GENE, 1997, 188 (01) :45-52
[3]   Massive parallel analysis of DNA-Hoechst 33258 binding specificity with a generic oligodeoxyribonucleotide microchip [J].
Drobyshev, AL ;
Zasedatelev, AS ;
Yershov, GM ;
Mirzabekov, AD .
NUCLEIC ACIDS RESEARCH, 1999, 27 (20) :4100-4105
[4]   Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization [J].
Dubiley, S ;
Kirillov, E ;
Lysov, Y ;
Mirzabekov, A .
NUCLEIC ACIDS RESEARCH, 1997, 25 (12) :2259-2265
[5]   Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips [J].
Fotin, AV ;
Drobyshev, AL ;
Proudnikov, DY ;
Perov, AN ;
MIrzabekov, AD .
NUCLEIC ACIDS RESEARCH, 1998, 26 (06) :1515-1521
[6]   Manual manufacturing of oligonucleotide, DNA, and protein microchips [J].
Guschin, D ;
Yershov, G ;
Zaslavsky, A ;
Gemmell, A ;
Shick, V ;
Proudnikov, D ;
Arenkov, P ;
Mirzabekov, A .
ANALYTICAL BIOCHEMISTRY, 1997, 250 (02) :203-211
[7]  
Hermanson GT, 1996, BIOCONJUGATE TECHNIQ, P785
[8]  
Khrapko K R, 1991, DNA Seq, V1, P375, DOI 10.3109/10425179109020793
[9]   Theoretical analysis of the kinetics of DNA hybridization with gel-immobilized oligonucleotides [J].
Livshits, MA ;
Mirzabekov, AD .
BIOPHYSICAL JOURNAL, 1996, 71 (05) :2795-2801
[10]   Protein microarrays for gene expression and antibody screening [J].
Lueking, A ;
Horn, M ;
Eickhoff, H ;
Büssow, K ;
Lehrach, H ;
Walter, G .
ANALYTICAL BIOCHEMISTRY, 1999, 270 (01) :103-111