Fluorescence polarization as a functional parameter in monitoring living cells: Theory and practice

被引：13

作者：

Deutsch, M ^{[1
]}

Tirosh, R

Kaufman, M

Zurgil, N

Weinreb, A

机构：

[1] Bar Ilan Univ, Biophys Interdisciplinary Ctr Res & Technol Cello, IL-52900 Ramat Gan, Israel

[2] Bar Ilan Univ, Jerome Schottenstein Ctr Early Detect Canc, IL-52900 Ramat Gan, Israel

来源：

JOURNAL OF FLUORESCENCE | 2002年 / 12卷 / 01期

关键词：

fluorescence; polarization; flow cytometry; static cytometry; excitation power density; photodamage; photo biostimulation; individual living cells;

D O I：

10.1023/A:1015355018449

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper.

引用

页码：25 / 44

页数：20

共 66 条

[1] Differential signaling by lymphocyte antigen receptors [J].