Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain

被引:93
作者
Hauptmann, G [1 ]
Gerster, T [1 ]
机构
[1] Univ Basel, Biozentrum, CH-4056 Basel, Switzerland
关键词
forebrain; diencephalon; prosomere; neuromere; longitudinal brain axis; alar/basal boundary; floor plate; roof plate; alar plate; basal plate; two-color whole-mount in situ hybridization;
D O I
10.1016/S0925-4773(99)00277-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
To shed light on the organization of the rostral embryonic brain of a lower vertebrate, we have directly compared the expression patterns of dlx, fgf, hh, hlx, otx, pas, POU, winged helix and wnt gene family members in the fore- and midbrain of the zebrafish. We show that the analyzed genes are expressed in distinct transverse and longitudinal domains and share expression boundaries at stereotypic positions within thr fore- and midbrain. Some of these shared expression boundaries coincide with morphological landmarks like the pathways of primary axon tracts. We identified a series of right transverse diencephalic domains suggestive of neuromeric subdivisions within the rostral brain. in addition, we identified four molecularly distinct longitudinal subdivisions and provide evidence for a strong bending of the longitudinal rostral brain axis at the cephalic flexure. our data suggest a strong conservation of early forebrain organization between lower and higher vertebrates. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:105 / 118
页数:14
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