A new matrix protein family related to the nacreous layer formation of Pinctada fucata

被引：285

作者：

Samata, T

Hayashi, N

Kono, M

Hasegawa, K

Horita, C

Akera, S

机构：

[1] Azabu Univ, Cell Biol Lab, Fac Environm Hlth, Sagamihara, Kanagawa 2298501, Japan

[2] Omgen Inc, Tokyo 1730031, Japan

[3] New Japan Alfo Inc, Atsugi, Kanagawa 2430811, Japan

[4] Tasaki Inst Marine Bio Res, Tokushima 77923, Japan

来源：

FEBS LETTERS | 1999年 / 462卷 / 1-2期

关键词：

biomineralization; nacreous layer; organic matrix; cDNA cloning; crystallization experiment;

D O I：

10.1016/S0014-5793(99)01387-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a new matrix protein family (N16) which is specific to the nacreous layer of the Japanese pearl oyster, Pinctada fucata, and have cloned and characterized the cDNAs coding for the components. Analysis of the deduced amino acid sequence revealed that N16 showed no definitive homology,vith other proteins. The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation without it, The aragonite crystals showed platy morphology different from those formed inorganically, and long intervals of incubation resulted in crystalline layers highly similar to the nacreous layer. (C) 1999 Federation of European Biochemical Societies.

引用

页码：225 / 229

页数：5

共 16 条

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