Cyanobacteria carrying an smt-lux transcriptional fusion as biosensors for the detection of heavy metal cations

被引：46

作者：

Erbe, JL ^{[1
]}

Adams, AC ^{[1
]}

Taylor, KB ^{[1
]}

Hall, LM ^{[1
]}

机构：

[1] UNIV ALABAMA, DEPT BIOCHEM & MOL GENET, BIRMINGHAM, AL 35294 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1996年 / 17卷 / 02期

关键词：

cyanobacteria; biosensor; heavy metal; lux;

D O I：

10.1007/BF01570047

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The metal-responsive smt operator/promoter region of Synechococcus PCC7942 was fused to the luxCDABE genes of Vibrio fischeri. Plasmid DNA (pJLE23) carrying this fusion conferred metal ion-inducible luminescence to transformed cyanobacteria. Synechococcus PCC7942 (pJLE23) was sensitive to ZnCl2 concentrations within a range of 0.5-4 mu M as demonstrated by induction of luminescence. Trace levels of CuSO4 and CdCl2 were also detected.

引用

页码：80 / 83

页数：4

共 13 条

[1] PLASMIDS FOR HEAVY-METAL RESISTANCE IN ALCALIGENES-EUTROPHUS CH34 - MECHANISMS AND APPLICATIONS
COLLARD, JM
CORBISIER, P
DIELS, L
DONG, Q
JEANTHON, C
MERGEAY, M
TAGHAVI, S
VANDERLELIE, D
WILMOTTE, A
WUERTZ, S
[J]. FEMS MICROBIOLOGY REVIEWS, 1994, 14 (04) : 405 - 414
[2] CORBISIER P, 1993, FEMS MICROBIOL LETT, V110, P231, DOI 10.1016/0378-1097(93)90471-D
[3] MEASURING GENE-EXPRESSION WITH LIGHT
ENGEBRECHT, J
SIMON, M
SILVERMAN, M
[J]. SCIENCE, 1985, 227 (4692) : 1345 - 1347
[4] METALLOREGULATION OF THE CYANOBACTERIAL SMT LOCUS - IDENTIFICATION OF SMTB BINDING-SITES AND DIRECT INTERACTION WITH METALS
ERBE, JL
TAYLOR, KB
HALL, LM
[J]. NUCLEIC ACIDS RESEARCH, 1995, 23 (13) : 2472 - 2478
[5] GEISELHART L, 1991, ANN NY ACAD SCI, V646, P53
[6] TRANSCRIPTION OF THE ESCHERICHIA-COLI-FLIC GENE IS REGULATED BY METAL-IONS
GUZZO, A
DIORIO, C
DUBOW, MS
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (08) : 2255 - 2259
[7] ISOLATION OF A PROKARYOTIC METALLOTHIONEIN LOCUS AND ANALYSIS OF TRANSCRIPTIONAL CONTROL BY TRACE-METAL IONS
HUCKLE, JW
MORBY, AP
TURNER, JS
ROBINSON, NJ
[J]. MOLECULAR MICROBIOLOGY, 1993, 7 (02) : 177 - 187
[8] KUHLEMEIER CJ, 1987, METHOD ENZYMOL, V153, P199
[9] SMTB IS A METAL-DEPENDENT REPRESSOR OF THE CYANOBACTERIAL METALLOTHIONEIN GENE SMTA - IDENTIFICATION OF A ZN INHIBITED DNA-PROTEIN COMPLEX
MORBY, AP
TURNER, JS
HUCKLE, JW
ROBINSON, NJ
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (04) : 921 - 925
[10] GENERIC ASSIGNMENTS, STRAIN HISTORIES AND PROPERTIES OF PURE CULTURES OF CYANOBACTERIA
RIPPKA, R
DERUELLES, J
WATERBURY, JB
HERDMAN, M
STANIER, RY
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1979, 111 (MAR): : 1 - 61

← 1 2 →