Interleukin 1-beta-induced protein kinase C-zeta activation is mimicked by exogenous phospholipase D

被引：45

作者：

Limatola, C ^{[1
]}

Barabino, B ^{[1
]}

Nista, A ^{[1
]}

Santoni, A ^{[1
]}

机构：

[1] UNIV ROMA LA SAPIENZA,DEPT EXPT MED & PATHOL,I-00161 ROME,ITALY

来源：

BIOCHEMICAL JOURNAL | 1997年 / 321卷

关键词：

D O I：

10.1042/bj3210497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin 1-beta (IL1-beta) is a pleiotropic cytokine that stimulates a number of signal transduction pathways in cells, leading to different cellular responses. In this study we investigated the signal transduction pathways activated by IL1-beta in two different human cell lines: RD/TE671, a rhabdomyosarcoma, and EJ, a bladder-derived carcinoma. We showed that this cytokine induced the activation of protein kinase C-zeta (PKC-zeta) and the accumulation of a putative physiological PKC-zeta activator, phosphatidic acid [Limatola, Schaap, Moolenaar and van Blitterswijk (1994) Biochem. J. 304, 1001-1008]. Exogenously supplied phospholipase D, which generated cellular phosphatidic acid, was able to mimic the cytokine effect, supporting the hypothesis that this lipid second messenger might contribute to cytokine-induced PKC-zeta activation. In addition, we show that IL1-beta stimulation of BOSC23 cells, transiently overexpressing PKC-zeta induced an increase in PKC-zeta autophosphorylation. These results give the first direct evidence that IL1-beta can activate this atypical PKC isoform and suggest that this enzyme might be involved in mediating some of the biological effects induced by IL1-beta.

引用

页码：497 / 501

页数：5

共 42 条

[1] PROTEIN KINASE-C-ZETA ISOFORM IS CRITICAL FOR MITOGENIC SIGNAL-TRANSDUCTION [J].

BERRA, E ;

DIAZMECO, MT ;

DOMINGUEZ, I ;

MUNICIO, MM ;

SANZ, L ;

LOZANO, J ;

CHAPKIN, RS ;

MOSCAT, J .

CELL, 1993, 74 (03) :555-563

[2] Evidence for a role of MEK and MAPK during signal transduction by protein kinase C zeta [J].

Berra, E ;

DiazMeco, MT ;

Lozano, J ;

Frutos, S ;

Municio, MM ;

Sanchez, P ;

Sanz, L ;

Moscat, J .

EMBO JOURNAL, 1995, 14 (24) :6157-6163

[3]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[4]

BURSTEN SL, 1991, J BIOL CHEM, V266, P20732

[5] PHOSPHOLIPASE-D - A DOWNSTREAM EFFECTOR OF ARF IN GRANULOCYTES [J].

COCKCROFT, S ;

THOMAS, GMH ;

FENSOME, A ;

GENY, B ;

CUNNINGHAM, E ;

GOUT, I ;

HILES, I ;

TOTTY, NF ;

TRUONG, Q ;

HSUAN, JJ .

SCIENCE, 1994, 263 (5146) :523-526

[6] ZETA-PKC INDUCES PHOSPHORYLATION AND INACTIVATION OF I-KAPPA-B-ALPHA IN-VITRO [J].

DIAZMECO, MT ;

DOMINGUEZ, I ;

SANZ, L ;

DENT, P ;

LOZANO, J ;

MUNICIO, MM ;

BERRA, E ;

HAY, RT ;

STURGILL, TW ;

MOSCAT, J .

EMBO JOURNAL, 1994, 13 (12) :2842-2848

[7] THE INTERLEUKIN-1 FAMILY - 10 YEARS OF DISCOVERY [J].

DINARELLO, CA .

FASEB JOURNAL, 1994, 8 (15) :1314-1325

[8]

DINARELLO CA, 1991, BLOOD, V77, P162

[9]

DOBROWSKY RT, 1992, J BIOL CHEM, V267, P5048

[10] INHIBITION OF PROTEIN-KINASE-C ZETA-SUBSPECIES BLOCKS THE ACTIVATION OF AN NF-KAPPA-B-LIKE ACTIVITY IN XENOPUS-LAEVIS OOCYTES [J].

DOMINGUEZ, I ;

SANZ, L ;

ARENZANASEISDEDOS, F ;

DIAZMECO, MT ;

VIRELIZIER, JL ;

MOSCAT, J .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (02) :1290-1295

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