A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana

被引:217
作者
Müller, A [1 ]
Düchting, P [1 ]
Weiler, EW [1 ]
机构
[1] Ruhr Univ Bochum, Fak Biol, Lehrstuhl Pflanzenphysiol, D-44780 Bochum, Germany
关键词
Arabidopsis (phytohormones); abscisic acid (GC-MS/MS analysis); chemical-ionization tandem mass spectrometry; indole-3-acetic acid (GC-MS/MS analysis); jasmonic acid (GC-MS/MS analysis); 12-oxo-phytodienoic acid (GC-MS/MS analysis); salicylic acid (GC-MS/MS analysis);
D O I
10.1007/s00425-002-0866-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A highly sensitive and accurate multiplex gas chromatography-tandem mass spectrometry. (GC-MS/MS) technique is reported for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid. The optimized setup allows the routine processing and analysis of up to 60 plant samples of between 20 and 200 mg of fresh weight per day. The protocol was designed and the equipment used was chosen to facilitate implementation of the method into other laboratories and to provide access to state-of-the-art analytical tools for the acidic phytohormones and related signalling molecules. Whole-plant organ-distribution maps for indole-3-acetic acid, abscisic acid, jasmonic acid, 12-oxo-phytodienoic acid and salicylic acid were generated for Arabidopsis thaliana (L.) Heynh. For leaves of A. thaliana, a spatial resolution of hormone quantitation down to approximately 2 mm(2) was achieved.
引用
收藏
页码:44 / 56
页数:13
相关论文
共 33 条
[1]   Vascular development: tracing signals along veins [J].
Berleth, T ;
Mattsson, J .
CURRENT OPINION IN PLANT BIOLOGY, 2000, 3 (05) :406-411
[2]  
BRODSCHELM W, 1995, THESIS MUNICH
[3]   CYTOKININ ACTION IS COUPLED TO ETHYLENE IN ITS EFFECTS ON THE INHIBITION OF ROOT AND HYPOCOTYL ELONGATION IN ARABIDOPSIS-THALIANA SEEDLINGS [J].
CARY, AJ ;
LIU, WN ;
HOWELL, SH .
PLANT PHYSIOLOGY, 1995, 107 (04) :1075-1082
[4]   SYNTHESIS OF 3 JASMIN CONSTITUENTS VIA A CENTRAL INTERMEDIATE [J].
DUBS, P ;
STUSSI, R .
HELVETICA CHIMICA ACTA, 1978, 61 (03) :990-997
[5]  
ECKER JR, 1994, ARABIDOPSIS, P485
[6]   A MICROSCALE TECHNIQUE FOR GAS-CHROMATOGRAPHY MASS-SPECTROMETRY MEASUREMENTS OF PICOGRAM AMOUNTS OF INDOLE-3-ACETIC-ACID IN PLANT-TISSUES [J].
EDLUND, A ;
EKLOF, S ;
SUNDBERG, B ;
MORITZ, T ;
SANDBERG, G .
PLANT PHYSIOLOGY, 1995, 108 (03) :1043-1047
[7]   Genetic interactions between ABA, ethylene and sugar signaling pathways [J].
Gazzarrini, S ;
McCourt, P .
CURRENT OPINION IN PLANT BIOLOGY, 2001, 4 (05) :387-391
[8]  
GMELIN R, 1961, ANN ACAD SCI FENN A2, V107, P2
[9]   WATER-STRESS-INDUCED CHANGES IN THE ABSCISIC-ACID CONTENT OF GUARD-CELLS AND OTHER CELLS OF VICIA-FABA L LEAVES AS DETERMINED BY ENZYME-AMPLIFIED IMMUNOASSAY [J].
HARRIS, MJ ;
OUTLAW, WH ;
MERTENS, R ;
WEILER, EW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2584-2588
[10]  
KNOFEL HD, 1988, Z NATURFORSCH C, V43, P29