Ca2+- and H+-dependent conformational changes of calbindin D28k

被引:40
作者
Berggård, T [1 ]
Silow, M [1 ]
Thulin, E [1 ]
Linse, S [1 ]
机构
[1] Univ Lund, Ctr Chem, Dept Biochem, S-22100 Lund, Sweden
关键词
D O I
10.1021/bi992394g
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Calbindin D-28k is a member of a large family of intracellular Ca2+ binding proteins characterized by EF-hand structural motifs. Some of these proteins are classified as Ca2+-sensor proteins, since they are involved in transducing intracellular Ca2+ signals by exposing a hydrophobic patch on the protein surface in response to Ca2+ binding. The hydrophobic patch serves as an interaction site for target enzymes. Other members of this group are classified as Ca2+ buffering proteins, because they remain closed after Ca2+ binding and participate in Ca2+ buffering and transport functions. ANS (8-anilinonaphthalene-1-sulfonic acid) binding and affinity chromatography on a hydrophobic column suggested that both the Ca2+-free and Ca2+-loaded form of calbindin D-28k have exposed hydrophobic surfaces. Since exposure of hydrophobic surface is unfavorable in the aqueous intracellular milieu, calbindin D-28k most likely interacts with other cellular components in vivo. A Ca2+-induced conformational change was readily detected by several optical spectroscopic methods. Thus, calbindin D-28k shares some of the properties of Ca2+-sensor proteins. However, the Ca2+-induced change in exposed hydrophobic surface was considerably less pronounced than that in calmodulin. The data also shows that calbindin D28k undergoes a rapid and reversible conformational change in response to a H+ concentration increase within the physiological pH range. The pH-dependent conformational change was shown to reside mainly in EF-hands 1-3. Urea-induced unfolding of the protein at pH 6, 7, and 8 showed that the stability of calbindin D-28k was increased in response to H+ in the range examined. The results suggest that calbindin D-28k may interact with targets in a Ca2+- and H+-dependent manner.
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页码:6864 / 6873
页数:10
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共 51 条
  • [1] Ataxia and altered dendritic calcium signaling in mice carrying a targeted null mutation of the calbindin D28k gene
    Airaksinen, MS
    Eilers, J
    Garaschuk, O
    Thoenen, H
    Konnerth, A
    Meyer, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (04) : 1488 - 1493
  • [2] Ca2+-binding stoichiometry of calbindin D-28k as assessed by spectroscopic analyses of synthetic peptide fragments
    Akerfeldt, KS
    Coyne, AN
    Wilk, RR
    Thulin, E
    Linse, S
    [J]. BIOCHEMISTRY, 1996, 35 (12) : 3662 - 3669
  • [3] PROTEIN STABILITY AND ELECTROSTATIC INTERACTIONS BETWEEN SOLVENT EXPOSED CHARGED SIDE-CHAINS
    AKKE, M
    FORSEN, S
    [J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1990, 8 (01): : 23 - 29
  • [4] DISTRIBUTION OF CALRETININ, CALBINDIN-D28K, AND PARVALBUMIN IN THE RAT THALAMUS
    ARAI, R
    JACOBOWITZ, DM
    DEURA, S
    [J]. BRAIN RESEARCH BULLETIN, 1994, 33 (05) : 595 - 614
  • [5] DISTRIBUTION OF CALMODULIN, CALBINDIN-D28K AND CALRETININ AMONG RAT OLFACTORY NERVE BUNDLES
    BASTIANELLI, E
    POCHET, R
    [J]. NEUROSCIENCE LETTERS, 1994, 169 (1-2) : 223 - 226
  • [6] CHEMICAL COMPOSITION, AFFINITY FOR CALCIUM, AND SOME RELATED PROPERTIES OF VITAMIN-D DEPENDENT CALCIUM-BINDING PROTEIN
    BREDDERMAN, PJ
    WASSERMAN, RH
    [J]. BIOCHEMISTRY, 1974, 13 (08) : 1687 - 1694
  • [7] CALCIUM BUFFERING PROPERTIES OF CALBINDIN-D(28K) AND PARVALBUMIN IN RAT SENSORY NEURONS
    CHARD, PS
    BLEAKMAN, D
    CHRISTAKOS, S
    FULLMER, CS
    MILLER, RJ
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1993, 472 : 341 - 357
  • [8] CALCIUM-BINDING BY CHICK CALRETININ AND RAT CALBINDIN-D28K SYNTHESIZED IN BACTERIA
    CHEUNG, WT
    RICHARDS, DE
    ROGERS, JH
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 215 (02): : 401 - 410
  • [9] STUDIES ON THE MODE OF ACTION OF CALCIFEROL .13. DEVELOPMENT OF A RADIOIMMUNOASSAY FOR VITAMIN-D-DEPENDENT CHICK INTESTINAL CALCIUM-BINDING PROTEIN AND TISSUE DISTRIBUTION
    CHRISTAKOS, S
    FRIEDLANDER, EJ
    FRANDSEN, BR
    NORMAN, AW
    [J]. ENDOCRINOLOGY, 1979, 104 (05) : 1495 - 1503
  • [10] VITAMIN D-DEPENDENT CALCIUM-BINDING PROTEINS - CHEMISTRY, DISTRIBUTION, FUNCTIONAL CONSIDERATIONS, AND MOLECULAR-BIOLOGY
    CHRISTAKOS, S
    GABRIELIDES, C
    RHOTEN, WB
    [J]. ENDOCRINE REVIEWS, 1989, 10 (01) : 3 - 26