Generation of colonic IgA-secreting cells in the caecal patch

被引：136

作者：

Masahata, Kazunori ^{[1
,2
]}

Umemoto, Eiji ^{[1
,3
]}

Kayama, Hisako ^{[1
,3
]}

Kotani, Manato ^{[4
]}

Nakamura, Shota ^{[5
]}

Kurakawa, Takashi ^{[1
]}

Kikuta, Junichi ^{[4
]}

Gotoh, Kazuyoshi ^{[5
]}

Motooka, Daisuke ^{[5
]}

Sato, Shintaro ^{[6
]}

Higuchi, Tomonori ^{[7
]}

Baba, Yoshihiro ^{[8
]}

Kurosaki, Tomohiro ^{[8
]}

Kinoshita, Makoto ^{[1
,3
]}

Shimada, Yosuke ^{[1
]}

Kimura, Taishi ^{[1
]}

Okumura, Ryu ^{[1
]}

Takeda, Akira ^{[1
]}

Tajima, Masaru ^{[9
]}

Yoshie, Osamu ^{[7
]}

Fukuzawa, Masahiro

Kiyono, Hiroshi

Fagarasan, Sidonia ^{[10
]}

Iida, Tetsuya ^{[5
,11
]}

Ishii, Masaru ^{[3
,4
]}

Takeda, Kiyoshi ^{[1
,3
]}

机构：

[1] Osaka Univ, Lab Immune Regulat, Dept Microbiol & Immunol, Grad Sch Med,WPI Immunol Frontier Res Ctr, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Med, Dept Pediat Surg, Suita, Osaka 5650871, Japan

[3] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Kawaguchi, Saitama 3320012, Japan

[4] Osaka Univ, WPI Immunol Frontier Res Ctr, Grad Sch Med, Dept Immunol & Cell Biol, Suita, Osaka 5650871, Japan

[5] Osaka Univ, Microbial Dis Res Inst, Genome Informat Res Ctr, Dept Infect Metagen, Suita, Osaka 5650871, Japan

[6] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Mucosal Immunol, Tokyo 1088639, Japan

[7] Kinki Univ, Fac Med, Dept Microbiol, Osaka 5898511, Japan

[8] Osaka Univ, WPI Immunol Frontier Res Ctr, Lab Lymphocyte Differentiat, Suita, Osaka 5650871, Japan

[9] Osaka Univ, Fac Med, Inst Expt Anim Sci, Suita, Osaka 5650871, Japan

[10] RIKEN, Ctr Integrat Med Sci, Lab Mucosal Immun, Yokohama, Kanagawa 2300045, Japan

[11] Osaka Univ, Microbial Dis Res Inst, Int Res Ctr Infect Dis, Lab Genom Res Pathogen Bacteria, Suita, Osaka 5650871, Japan

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

基金：

日本科学技术振兴机构;

关键词：

CD103(+) DENDRITIC CELLS; BETA T-CELLS; FOLLICLE FORMATION; RECEPTOR; APPENDIX; BACTERIA; REQUIREMENT; MICROBIOTA; IMMUNITY; ANTIGEN;

D O I：

10.1038/ncomms4704

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

引用

页数：13

共 52 条

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