Regulation of actin dynamics by WASP and WAVE family proteins

被引:239
作者
Stradal, TEB
Rottner, K
Disanza, A
Confalonieri, S
Innocenti, M
Scita, G [1 ]
机构
[1] Ist FIRC Oncol Mol, I-20134 Milan, Italy
[2] German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany
[3] Ist Europeo Oncol, Dept Expt Oncol, I-20141 Milan, Italy
关键词
D O I
10.1016/j.tcb.2004.04.007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Signal-dependent regulation of actin dynamics is essential for a variety of cellular processes, including formation of the membrane protrusions required for cell locomotion. Wiskott-Aldrich syndrome protein (WASP), neural (N)-WASP and WASP family verprolin-homologous (WAVE, also named Scar) proteins are thought to play a role in these processes by relaying activation signals from small GTPases, such as Cdc42 and Rac, to the actin-nucleating complex Arp2/3. Although much biochemical and structural biological work has defined the paradigms through which WASP and N-WASP regulation is achieved, only recently have the mechanisms that control WAVE proteins begun to be clarified. WAVE proteins assemble into macromolecular complexes, which are essential for the regulation of WAVE nucleation-promoting activity, dynamic localization and stability. In this article, we discuss recent studies that highlight novel modalities through which WAVE proteins are regulated and, in turn, mediate the site-directed actin polymerization required for membrane protrusion, thus enabling cell motility.
引用
收藏
页码:303 / 311
页数:9
相关论文
共 82 条
[1]   Actin filament uncapping localizes to ruffling lamellae and rocketing vesicles [J].
Allen, PG .
NATURE CELL BIOLOGY, 2003, 5 (11) :972-979
[2]   SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development [J].
Bear, JE ;
Rawls, JF ;
Saxe, CL .
JOURNAL OF CELL BIOLOGY, 1998, 142 (05) :1325-1335
[3]   Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling [J].
Ben-Yaacov, S ;
Le Borgne, R ;
Abramson, I ;
Schweisguth, F ;
Schejter, ED .
JOURNAL OF CELL BIOLOGY, 2001, 152 (01) :1-13
[4]   PIR121 regulates pseudopod dynamics and SCAR activity in Dictyostelium [J].
Blagg, SL ;
Stewart, M ;
Sambles, C ;
Insall, RH .
CURRENT BIOLOGY, 2003, 13 (17) :1480-1487
[5]   Kette regulates actin dynamics and genetically interacts with Wave and Wasp [J].
Bogdan, S ;
Klämbt, C .
DEVELOPMENT, 2003, 130 (18) :4427-4437
[6]   Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP [J].
Cory, GOC ;
Cramer, R ;
Blanchoin, L ;
Ridley, AJ .
MOLECULAR CELL, 2003, 11 (05) :1229-1239
[7]   Phosphorylation of tyrosine enhances the ability of WASp to stimulate actin polymerization and filopodium formation [J].
Cory, GOC ;
Garg, R ;
Cramer, R ;
Ridley, AJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (47) :45115-45121
[8]   Cell motility - Braking WAVEs [J].
Cory, GOC ;
Ridley, AJ .
NATURE, 2002, 418 (6899) :732-733
[9]  
Dahl JP, 2003, J NEUROSCI, V23, P3343
[10]   ISOLATION OF A NOVEL GENE MUTATED IN WISKOTT-ALDRICH SYNDROME [J].
DERRY, JMJ ;
OCHS, HD ;
FRANCKE, U .
CELL, 1994, 78 (04) :635-644