Oxygen-dependent H2O2 production by Rubisco

被引：65

作者：

Kim, K ^{[1
]}

Portis, AR ^{[1
]}

机构：

[1] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

来源：

FEBS LETTERS | 2004年 / 571卷 / 1-3期

关键词：

oxidation; carbon metabolism; photosynthesis;

D O I：

10.1016/j.febslet.2004.06.064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxygen and ribulose-1,5-bisphosphate dependent, H2O2 production was observed with several wild type Rubisco enzymes using a sensitive assay. H2O2 and D-glycero-2,3-pentodiulose-1,5-bisphosphate, a known and potent inhibitor of Rubisco activity, are predicted products arising from elimination of H2O2 from a peroxyketone intermediate, specific to oxygenase activity. Parallel assays using varying CO2 and O-2 concentrations revealed that the partitioning to H2O2 during O-2 consumption by spinach Rubisco was constant at 1/260-1/270. High temperature (38 degreesC), which reduces Rubisco specificity for CO2 versus O-2, increased the rates of H2O2 production and O-2 consumption, resulting in a small increase in partitioning to H2O2 (1/210). Two Rubiscos with lower specificity than spinach exhibited greater partitioning to H2O2 during catalysis: Chlamydomonas reinhardtii (1/200); and Rhodospirillum rubrum (1/150). (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：124 / 128

页数：5

共 24 条

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