High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells

被引：10

作者：

Cui, Xiao-Nan ^{[2
]}

Tang, Jian-Wu ^{[1
]}

Song, Bo ^{[1
]}

Wang, Bo ^{[1
]}

Chen, Shan-Yan ^{[3
]}

Hou, Li ^{[1
]}

机构：

[1] Dalian Med Univ, Dept Pathol, Dalian 116044, Liaoning, Peoples R China

[2] Dalian Med Univ, Dept Oncol, Affiliated Hosp 1, Dalian 116011, Liaoning, Peoples R China

[3] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 430074, Hubei, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2009年 / 15卷 / 48期

基金：

中国国家自然科学基金;

关键词：

Osteoglycin; Transfection; Hepatocellular carcinoma; Neoplasm metastasis; Genes; Gelatinases; NECROSIS-FACTOR-ALPHA; MATRIX METALLOPROTEINASES; EXTRACELLULAR-MATRIX; INVASIVE ABILITY; BOVINE MIMECAN; DEFICIENT MICE; BREAST-CANCER; METASTASIS; GROWTH; MICROENVIRONMENT;

D O I：

10.3748/wjg.15.6117

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells. METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis. RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo. CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression. (C) 2009 The WJG Press and Baishideng. All rights reserved

引用

页码：6117 / 6122

页数：6

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