Characterization of the natural chemical and osmotic environment of early wheat embryogenesis

Soluble carbohydrates, amino acids, and major inorganic ions were quantified and compared,vith the total osmolarity of the ovule, Most of the analyzed components increased rapidly in concentration, reaching a peak at 1 day post anthesis (DPA) and decreasing quickly 1 day later. Within this time period, the osmolarity decreased from 1.49 to 1.18 MPa, The osmolarity then increased to 1.39 MPa at 4 DPA, and yet the overall concentration of analyzed molecules decreased, At 1 DPA, inorganic ions were observed at levels that could produce a high osmolarity (1.11 MPa), followed by the carbohydrates (0.37 MPa) and amino acids (0.07 MPa), Maltose was detected in ovules only from 0 to 2 DPA, Inositol was abundant in the ovules on the day of anthesis, but decreased to non-detectable levels after 4 DPA, This suggested that ovules allow the rapid entry of solutes into the syncytial endosperm after fertilization, but are osmotically buffered through the increase and decrease of partly identified substances. Specific gravity tests on the liquid squeezed from young endosperm supported this new hypothesis, Osmolarity in ovules seems only indirectly related to morphogenetic regulation mechanisms, and it may play a lesser role than the fluctuations of certain specific substances, The observed chemical changes offer insights into the rapidly varying nutritional needs of proembryos. Thus, this research yielded a useful complex amino acid formula, derived from knowledge of the natural environment of the proembryo, and also an improved proembryo and ovule culture medium.