Purification and characterization of ferredoxin from Hydrogenobacter thermophilus strain TK-6

被引：7

作者：

Ishii, M ^{[1
]}

Ueda, Y ^{[1
]}

Yoon, KS ^{[1
]}

Igarashi, Y ^{[1
]}

Kodama, T ^{[1
]}

机构：

[1] UNIV TOKYO, DEPT BIOTECHNOL, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 09期

关键词：

ferredoxin; hydrogen bacterium; reductive TCA cycle;

D O I：

10.1271/bbb.60.1513

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ferredoxin was purified from cells of Hydrogenobacter thermophilus strain TK-6. Purification was performed aerobically by the addition of octyl-beta-glucoside to the buffers, The purified ferredoxin had a molecular mass of 13,000 and contained a [4Fe-4S] cluster. The protein had a long stretch at the N-terminal region; however, the sequence was not similar to the sequences of ferredoxins with a long stretch from Archaebacteria.

引用

页码：1513 / 1515

页数：3

共 15 条

[1] PURIFICATION AND CHARACTERIZATION OF PYRUVATE FERREDOXIN OXIDOREDUCTASE FROM THE HYPERTHERMOPHILIC ARCHAEON PYROCOCCUS-FURIOSUS [J].

BLAMEY, JM ;

ADAMS, MWW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1161 (01) :19-27

[2] CHARACTERIZATION OF AN ANCESTRAL TYPE OF PYRUVATE FERREDOXIN OXIDOREDUCTASE FROM THE HYPERTHERMOPHILIC BACTERIUM, THERMOTOGA-MARITIMA [J].

BLAMEY, JM ;

ADAMS, MWW .

BIOCHEMISTRY, 1994, 33 (04) :1000-1007

[3] PURIFICATION AND PARTIAL CHARACTERIZATION OF A PYRUVATE OXIDOREDUCTASE FROM THE PHOTOSYNTHETIC BACTERIUM RHODOSPIRILLUM-RUBRUM GROWN UNDER NITROGEN-FIXING CONDITIONS [J].

BROSTEDT, E ;

NORDLUND, S .

BIOCHEMICAL JOURNAL, 1991, 279 :155-158

[4]

GEHRING U, 1972, J BIOL CHEM, V247, P6963

[5] AMINO-ACID-SEQUENCE OF 2FE-2S FERREDOXIN FROM AN EXTREME HALOPHILE, HALOBACTERIUM OF THE DEAD-SEA [J].

HASE, T ;

WAKABAYASHI, S ;

MATSUBARA, H ;

MEVARECH, M ;

WERBER, MM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 623 (01) :139-145

[6] PURIFICATION AND CHARACTERIZATION OF ATP - CITRATE LYASE FROM HYDROGENOBACTER-THERMOPHILUS TK-6 [J].

ISHII, M ;

IGARASHI, Y ;

KODAMA, T .

JOURNAL OF BACTERIOLOGY, 1989, 171 (04) :1788-1792

[7] ALTERNATIVE FORM OF THE DICLUSTER FERREDOXIN FROM THE THERMOACIDOPHILIC ARCHAEON, SULFOLOBUS SP STRAIN-7 [J].

IWASAKI, T ;

FUJII, T ;

WAKAGI, T ;

OSHIMA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 206 (02) :563-569

[8] HYDROGENOBACTER-THERMOPHILUS GEN-NOV, SP-NOV, AN EXTREMELY THERMOPHILIC, AEROBIC, HYDROGEN-OXIDIZING BACTERIUM [J].

KAWASUMI, T ;

IGARASHI, Y ;

KODAMA, T ;

MINODA, Y .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1984, 34 (01) :5-10

[9] PYRUVATE, FERREDOXIN OXIDOREDUCTASE FROM THE SULFATE-REDUCING ARCHAEOGLOBUS-FULGIDUS - MOLECULAR COMPOSITION, CATALYTIC PROPERTIES, AND SEQUENCE ALIGNMENTS [J].

KUNOW, J ;

LINDER, D ;

THAUER, RK .

ARCHIVES OF MICROBIOLOGY, 1995, 163 (01) :21-28

[10]

LOVENBERG W, 1963, J BIOL CHEM, V238, P3899

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