Lipid Incorporation Inhibits Src-Dependent Assembly of Fibronectin and Type I Collagen by Vascular Smooth Muscle Cells

被引:58
作者
Frontini, Matthew J. [1 ,3 ]
O'Neil, Caroline [1 ]
Sawyez, Cynthia [1 ]
Chan, Bosco M. C. [5 ]
Huff, Murray W. [1 ,2 ,3 ]
Pickering, J. Geoffrey [1 ,2 ,3 ,4 ]
机构
[1] London Hlth Sci Ctr, Robarts Res Inst, London, ON N6A 5A5, Canada
[2] Univ Western Ontario, Dept Med Cardiol, London, ON, Canada
[3] Univ Western Ontario, Dept Biochem, London, ON, Canada
[4] Univ Western Ontario, Dept Med Biophys, London, ON, Canada
[5] Univ Western Ontario, Dept Microbiol & Immunol, Schulich Sch Med & Dent, London, ON, Canada
基金
加拿大健康研究院;
关键词
smooth muscle cell; lipids; collagen; fibronectin; Src; integrin; MATRIX ADHESIONS; ATHEROSCLEROTIC PLAQUE; ENDOPLASMIC-RETICULUM; ACTIN-FILAMENTS; CHOLESTEROL; POLYMERIZATION; ACCUMULATION; INTEGRIN; ALPHA(2)BETA(1); LIPOPROTEINS;
D O I
10.1161/CIRCRESAHA.108.187302
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
A vital role of vascular smooth muscle cells (SMCs) is to stabilize the artery wall by elaborating fibrils of type I collagen. This is especially important in atherosclerotic lesions. However, SMCs in these lesions can be laden with lipids and the impact of this modification on collagen fibril formation is unknown. To address this, we converted human vascular SMCs to a foam cell state by incubating them with either LDL or VLDL. Biochemical markers of a SMC phenotype were preserved. However, microscopic tracking revealed a profound perturbation in the ability of the cells to assemble collagen fibrils, reducing assembly by up to 79%. This dysfunction was mirrored by an inability of smooth muscle foam cells to assemble fibronectin. Lipid-loaded SMCs did not display a generalized defect in the actin cytoskeleton and the formation of vinculin-containing focal adhesion complexes was preserved. However, lipid-loaded SMCs were unable to assemble fibrillar adhesion complexes and clustering of tensin and alpha 5 beta 1 integrin was disordered. Moreover, phosphorylation of tensin, required for fibrillar adhesion complex formation, was suppressed by up to 57%, with a concomitant decrease in activation of Src and FAK and restriction of activated Src to the cell edges. Forced activation of Src-FAK signaling in lipid-engorged SMCs rescued both fibrillar adhesion formation and fibrillogenesis. We conclude that lipid accumulation by SMCs disables the machinery for collagen and fibronectin assembly. This previously unknown relationship between atherogenic lipids and integrin-based signaling could underlie plaque vulnerability. (Circ Res. 2009;104:832-841.)
引用
收藏
页码:832 / U40
页数:24
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