Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection

被引：32

作者：

Chung, Woo Chul ^{[1
]}

Jung, Sung Hoon ^{[1
]}

Oh, Jung Hwan ^{[1
]}

Kim, Tae Ho ^{[1
]}

Cheung, Dae Young ^{[1
]}

Kim, Byung Wook ^{[1
]}

Kim, Sung Soo ^{[1
]}

Kim, Jin Il ^{[1
]}

Sin, Eun Young ^{[2
]}

机构：

[1] Catholic Univ Korea, Dept Internal Med, Seoul 130709, South Korea

[2] Catholic Univ Korea, St Vincent Hosp, Res Inst, Suwon 442723, South Korea

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2014年 / 20卷 / 21期

关键词：

Helicobacter pylori; Diagnosis; Dual-priming oligonucleotide-based multiplex polymerase chain reaction; BLEEDING PEPTIC-ULCER; 23S RIBOSOMAL-RNA; CLARITHROMYCIN RESISTANCE; ANTIBIOTIC-RESISTANCE; ERADICATION; MUTATIONS; DIAGNOSIS;

D O I：

10.3748/wjg.v20.i21.6547

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

AIM: To investigate whether tissue samples processed by the rapid urease test (RUT) kit are suitable for dual-priming oligonucleotide-based multiplex polymerase chain reaction (DPO-PCR) to detect Helicobacter pylori (H. pylori). METHODS: A total of 54 patients with specific gastrointestinal symptom were enrolled in this study. During endoscopy, gastric biopsy specimens were taken for histology, RUT, and DPO-PCR. DPO-PCR was performed on gastric biopsy samples and tissue samples that were analyzed by RUT at 2 separate institutes. In detecting H. pylori, the concordance rate of the DPO-PCR tests between the tissue samples that had been submitted to RUT and the gastric biopsy samples was investigated. RESULTS: H. pylori co-occurred with 76.0% (19/25) of gastric ulcers, 64.3% (9/14) of duodenal ulcers, and 33.3% (4/12) of gastritis cases. H. pylori infection was found in 100% (3/3) of the patients with both gastric and duodenal ulcers. Overall, H. pylori was detected in 35 of 54 (64.8%) patients. The diagnostic sensitivities of histology, RUT, and DPO-PCR were 85.7% (30/35), 74.3% (26/35), and 97.1% (34/35), respectively (P = 0.02). The positive predictive value (PPV) of DPO-PCR was 94.4%, whereas the negative predictive value (NPV) was 94.7%. In the rapid urease test (CLOtest)-negative cases, the frequency of positive DPO-PCR and histologic results was 20.0% (7/35). The concordance rate of the DPO-PCR tests between the tissue samples from the RUT kit and the gastric biopsy samples was 94.4% (51/54). The rate of DPO-PCR and silver stain positivity in the RUT-negative cases was 20.0% (7/35). CONCLUSION: In diagnosing H. pylori infection, DPO-PCR can be performed on tissue samples that have been processed by the RUT kit. Particularly, in patients with RUT-negative results, DPO-PCR on these tissue samples could be helpful in detecting of H. pylori infection. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

引用

页码：6547 / 6553

页数：7

共 24 条

[1]

Validation of a Fluorescence In Situ Hybridization Method Using Peptide Nucleic Acid Probes for Detection of Helicobacter pylori Clarithromycin Resistance in Gastric Biopsy Specimens [J].