Presence of methyl branched fatty acids and saturated hydrocarbons in botryococcene producing strain of Botqococcus braunii

Botryococcus braunii (N-836) produced 60 - 73 % hydrocarbons on dry weight basis, of which C-34 botryococcene was found to be the major hydrocarbon, constituting about 50 - 76 % of total content throughout the experimental studies. Major fatty acids present in this organism were C18:1 and C16:0. Saturated hydrocarbons like docosane, hexacosane and heptacosane were also found to be produced by the organism. Methyl branched fatty acids, were identified as 16-methyl heptadecanoic and 5, 9, 13 - trimethyl tetradecanoic acids by GC-MS. Maximum hydrocarbon accumulation was observed during third week of its growth.