Stimulation of vaccinia virion DNA helicase 18R, but not A18R, by a vaccinia core protein L4R, an ssDNA binding protein

被引:7
作者
Bayliss, CD [1 ]
Wilcock, D [1 ]
Smith, GL [1 ]
机构
[1] UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL,OXFORD OX1 3RE,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1099/0022-1317-77-11-2827
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Four DNA-dependent ATPases were purified from vaccinia virions and tested for DNA helicase activity on two dsDNA substrates. ATPases D6R and D11L were inactive on both substrates, A18R unwound the substrate with a short 20 bp duplex region and 18R unwound both substrates. In addition, the 18R protein was stimulated to unwind longer DNA duplexes by a 25 kDa protein purified from vaccinia virions, representing the cleaved product of the L4R gene, an ssDNA binding protein. Purified recombinant 25 kDa L4R protein also stimulated 18R DNA helicase activity and maximum activity was observed only when there were < 13 nucleotides of DNA per molecule of L4R protein. The DNA helicase activity of the A18R protein was not stimulated by either recombinant 25 kDa L4R protein or by an E. coli ssDNA binding protein.
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收藏
页码:2827 / 2831
页数:5
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