The genome-wide screening of yeast deletion mutants to identify the genes required for tolerance to ethanol and other alcohols

被引：125

作者：

Fujita, Katsuhide

Matsuyama, Akinobu

Kobayashi, Yoshinori

Iwahashi, Hitoshi

机构：

[1] Human Stress Signal Res Ctr, AIST, Tsukuba, Ibaraki 3058566, Japan

[2] JBA Daicel Chem Ind, Tsukuba, Ibaraki, Japan

来源：

FEMS YEAST RESEARCH | 2006年 / 6卷 / 05期

关键词：

yeast deletion clones; ethanol; alcohol; V-ATPase; Saccharomyces cerevisiae;

D O I：

10.1111/j.1567-1364.2006.00040.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A set of homozygous diploid deletion mutants of the yeast Saccharomyces cerevisiae was screened for the genes required for tolerance to aliphatic alcohols. The screen identified 137, 122 and 48 deletion mutants sensitive to ethanol, 1-propanol and 1-pentanol, respectively. A number of the genes required for ethanol tolerance were those also required for tolerance to other alcohols. Numerous mutants with defective genes encoding for vacuolar H+-ATPase (V-ATPase) were cosensitive to these alcohols. A global screening approach of yeast deletion library mutants was useful in elucidating the mechanisms of alcohol tolerance based on different lipophilicities.

引用

页码：744 / 750

页数：7

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