4-Hydroxynaphthyl-1-phosphate as a substrate for alkaline phosphatase and its use in sandwich immunoassay

被引：19

作者：

Másson, M ^{[1
]}

Haruyama, T ^{[1
]}

Kobatake, E ^{[1
]}

Aizawa, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 402卷 / 1-2期

关键词：

4-hydroxy-naphthyl-1-phosphate; immunoassay; alkaline phosphatase; amperometry;

D O I：

10.1016/S0003-2670(99)00499-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The synthesis and the use of a new substrate, 4-hydroxy-naphthyl-1-phosphate (HNP), for the amperometric detection of alkaline phosphatase activity is described. The product of the enzymatic hydrolysis of HNP was dihydroxy naphthalene (DHN). DHN was rapidly oxidized in air to give naphthoquinone (NQ), which was measured in amperometric flow injection analysis (AFIA) at 300 mV versus Ag/AgCl. DHN standards could be measured at a 60 nM detection limit. There was a linear response to the enzyme with a 300 fM detection limit, which was equivalent to 6 attomole for each injection. The measurement range for human IgG, in an alkaline phosphatase amplified sandwich immunoassay with amperometric, was 1-1000 ng/ml. (C)1999 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 35

页数：7

共 19 条

[1] PRINCIPLES AND APPLICATIONS OF ELECTROCHEMICAL AND OPTICAL BIOSENSORS [J].