Production and characterization of monoclonal antibodies for aflatoxin B1

被引：53

作者：

Devi, KT

Mayo, MA

Reddy, KLN

Delfosse, P

Reddy, G

Reddy, SV

Reddy, DVR ^{[1
]}

机构：

[1] Int Crops Res Inst Semi Arid Trop, GREP, Patancheru 502324, Andhra Pradesh, India

[2] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland

[3] Osmania Univ, Hyderabad 500007, Andhra Pradesh, India

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 05期

关键词：

D O I：

10.1046/j.1472-765X.1999.00685.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, one antibody was highly specific to B1, four antibodies reacted equally strongly with B1, G1 and weakly with B2. Another four reacted strongly with B1 and weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2. Interestingly all the 10 antibodies showed little or no cross-reaction with G2.

引用

页码：284 / 288

页数：5

共 11 条

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