The carbonyl group of glutamic acid-795 is essential for gastric H+,K+-ATPase activity

被引:26
作者
Hermsen, HPH [1 ]
Koenderink, JB [1 ]
Swarts, HGP [1 ]
De Pont, JJHHM [1 ]
机构
[1] Univ Nijmegen, Dept Biochem, Inst Cellular Signaling, NL-6500 HB Nijmegen, Netherlands
关键词
D O I
10.1021/bi991396y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To study the role of Glu(795), present in the fifth transmembrane domain of the alpha-subunit of gastric H+,K+-ATPase, several mutants were generated and expressed in Sf9) insect cells. The E795Q mutant had rather similar properties as the wild-type enzyme. The apparent affinity for K+ in both the ATPase reaction and the dephosphorylation of the phosphorylated intermediate was even slightly enhanced. This indicates that the carbonyl group of Glu(795) is sufficient for enzymatic activity. This carbonyl group, however, has to be at a particular position with respect to the other liganding groups, since the E795D and E795N mutants showed a strongly reduced ATPase activity, a lowered apparent K+ affinity, and a decreased steady-state phosphorylation level. In the absence of a carbonyl residue at position 795, the K+ sensitivity was either strongly decreased (E795A) or completely absent (E795L). The mutant E795L, however, showed a SCH 28080 sensitive ATPase activity in the absence of K+, as well as an enhanced spontaneous dephosphorylation rate, that could not be further enhanced by K+, suggesting that this mutant. mimicks the filled K+ binding pocket. The results indicate that the Glu(795) residue is involved in K+ stimulated ATPase activity and K+-induced dephosphorylation of the phosphorylated intermediate. Glu(795) might also be involved in H+ binding during the phosphorylation step, since the mutants E795N, E795D, and E795A showed a decrease in the phosphorylation rate as well as in the apparent ATP affinity in the phosphorylation reaction. This indicates that Glu(795) is not only involved in K+ but might also play a role in H+ binding.
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页码:1330 / 1337
页数:8
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