Chondroitin sulfate disrupts axon pathfinding in the optic tract and alters growth cone dynamics

被引:34
作者
Walz, A
Anderson, RB
Irie, A
Chien, CB
Holt, CE [1 ]
机构
[1] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[2] Rockefeller Univ, New York, NY 10021 USA
[3] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England
[4] Univ Utah, Med Ctr, Dept Neurobiol & Anat, Salt Lake City, UT 84132 USA
来源
JOURNAL OF NEUROBIOLOGY | 2002年 / 53卷 / 03期
关键词
glycosaminoglycans; proteoglycans; axon guidance; neurite extension; retinotectal pathway;
D O I
10.1002/neu.10113
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Little is known about the cues that guide retinal axons across the diencephalon en route to their midbrain target, the optic tectum. Here we show that chondroitin sulfate proteoglycans are differentially expressed within the diencephalon at a time when retinal axons are growing within the optic tract. Using exposed brain preparations, we show that the addition of exogenous chondroitin sulfate results in retinal pathfinding errors. Retinal axons disperse widely from their normal trajectory within the optic tract and extend aberrantly into inappropriate regions of the forebrain. Time-lapse analysis of retinal growth cone dynamics in vivo shows that addition of exogenous chondroitin sulfate causes intermittent stalling and increases growth cone complexity. These results suggest that chondroitin sulfate may modulate the guidance of retinal axons as they grow through the diencephalon towards the optic tectum. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:330 / 342
页数:13
相关论文
共 82 条
[1]   Chondroitin sulfates modulate axon guidance in embryonic Xenopus brain [J].
Anderson, RB ;
Walz, A ;
Holt, CE ;
Key, B .
DEVELOPMENTAL BIOLOGY, 1998, 202 (02) :235-243
[2]   SPATIAL INTERRELATIONSHIPS BETWEEN PROTEOGLYCANS AND EXTRACELLULAR-MATRIX PROTEINS IN CELL-CULTURES [J].
AVNUR, Z ;
GEIGER, B .
EXPERIMENTAL CELL RESEARCH, 1985, 158 (02) :321-332
[3]   Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish [J].
Becker, CG ;
Becker, T .
JOURNAL OF NEUROSCIENCE, 2002, 22 (03) :842-853
[4]  
Birgbauer E, 2000, DEVELOPMENT, V127, P1231
[5]  
BOVOLENTA P, 1987, J NEUROSCI, V7, P1447
[6]   UP-REGULATION OF A CHONDROITIN SULFATE EPITOPE DURING REGENERATION OF MOUSE SCIATIC-NERVE - EVIDENCE THAT THE IMMUNOREACTIVE MOLECULES ARE RELATED TO THE CHONDROITIN SULFATE PROTEOGLYCANS DECORIN AND VERSICAN [J].
BRAUNEWELL, KH ;
MARTINI, R ;
LEBARON, R ;
KRESSE, H ;
FAISSNER, A ;
SCHMITZ, B ;
SCHACHNER, M .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1995, 7 (04) :792-804
[7]   MULTIPLE FACTORS GOVERN INTRARETINAL AXON GUIDANCE - A TIME-LAPSE STUDY [J].
BRITTIS, PA ;
SILVER, J .
MOLECULAR AND CELLULAR NEUROSCIENCE, 1995, 6 (05) :413-432
[8]   CHONDROITIN SULFATE AS A REGULATOR OF NEURONAL PATTERNING IN THE RETINA [J].
BRITTIS, PA ;
CANNING, DR ;
SILVER, J .
SCIENCE, 1992, 255 (5045) :733-736
[9]   Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones [J].
Campbell, DS ;
Regan, AG ;
Lopez, JS ;
Tannahill, D ;
Harris, WA ;
Holt, CE .
JOURNAL OF NEUROSCIENCE, 2001, 21 (21) :8538-8547
[10]  
CAUDY M, 1986, J NEUROSCI, V6, P364